HGV NS_3 cDNA片段的表达及其免疫原性的研究  

Expression of NS 3 cDNA of HGV in Escherichia Coli and the Antogencity of the Expressed Protein

作　　者：李小洁[1,2] 马东晖[1,2] 马辉文[1,2] 童立恒 

机构地区：[1]武汉大学生命科学学院 [2]武汉市生物技术研究开发中心

出　　处：《武汉大学学报（自然科学版）》1999年第2期238-242,共5页Journal of Wuhan University(Natural Science Edition)

基　　金：武汉市科委资助项目

摘　　要：一段长度为１３２７ｂｐ的庚型肝炎病毒ｃＤＮＡ在大肠杆菌ＢＬ２１（ＤＥ３）菌株中得到表达．此ｃＤＮＡ被插入到噬粒ｐＧＥＭＤ中，位于编码大肠杆菌ＲＮＡ聚合酶σ７０－因子氨基端８个氨基酸残基的ＤＮＡ序列的下游，并与σ７０－因子处于同一阅读框．表达的含有ＨＧＶ蛋白质的融合蛋白达到宿主菌细胞蛋白总量的百分之十．免疫印迹实验证明。A 1327 base pair cDNA localized to the putative NS3 region of HGV genome was expressed in Escherichia coli BL 21 (DE 3). The cDNA fragment was inserted into a phagemid pGEMD, a derivative of pGEMEX 1(from Promega), at the downstream of the 5' terminal DNA sequence encoding the initial eight amino acids of σ 70 factor of E. coli RNA polymerase in the same reading frame with the gene of σ 70 factor. The amount of the expressed fusion protein was ten per cent of total cellular protein of the host cells.The expressed HGV protein could be specifically recognized with the sera from the patient infected by HGV.

关 键 词：庚肝病毒 基因表达 抗原性 免疫原性 病毒性肝炎 

分 类 号：R512.63[医药卫生—内科学] R373.21[医药卫生—临床医学]