奥卡西平对戊四氮诱导癫大鼠神经元凋亡的影响  

Effects of Oxcarbazepine on Apoptosis of Hippocampus Neuron in Epileptic Rats Induced by Pentylenetetrazol

作　　者：王克玲[1] 张梅杰[1] 施荣富[1] 唐红霞[1] 孙素真[1] 王丽辉[1] 

机构地区：[1]河北省儿童医院神经内科,石家庄050031

出　　处：《实用儿科临床杂志》2010年第14期1091-1093,共3页Journal of Applied Clinical Pediatrics

摘　　要：目的探讨奥卡西平对戊四氮致大鼠海马神经元凋亡的影响。方法 4周龄大鼠84只,随机分为4组:A组予9 g.L-1盐水(18只),B组予奥卡西平(18只),C组致后予9 g.L-1盐水(24只),D组致后予奥卡西平(24只)。C组、D组连续5 d腹腔注射戊四氮60 mg.kg-1.d-1,根据Racine分级观察大鼠发作情况,连续5 d出现Ⅳ～Ⅴ级癫发作,每次持续3～5 min为模型制备成功。第6天起,A组、C组腹腔注射9 g.L-1盐水,B组、D组予奥卡西平80 mg.kg-1.d-1灌胃。于喂药满9 d、15 d、21 d,处死动物取脑,做石蜡块,行HE、免疫组织化学染色。观察各组大鼠脑海马区神经细胞凋亡情况。结果 1.HE染色:光镜下,A组、B组脑组织形态学未见明显差别。C组可见细胞空泡样变性,胞膜内陷。D组可见大量凋亡细胞,细胞层数增多。2.Bcl-2表达:A组、B组大鼠海马区均有Bcl-2少量表达,在9 d、15 d、21 d时间点,组间比较差异均无统计学意义;C组Bcl-2阳性细胞数在9 d、15 d、21 d较A组、B组增多;D组Bcl-2的表达在9 d、15 d、21 d时较其他3组均显著增多;C组、D组Bcl-2因子早期显著表达后,迅速下降,但在21 d时仍显著高于A组、B组。3.Bax表达:A组、B组、D组3组大鼠海马区均有Bax表达,组间比较、组内各个时间点比较差异均无统计学意义。C组Bax阳性细胞数表达在9 d、15 d较其他3组均增多,差异有统计学意义,而在21 d时与上述3组比较无统计学差异。结论奥卡西平能减轻癫所致的海马部位神经元损伤,促进凋亡因子Bcl-2表达,抑制Bax表达。对大鼠海马区神经元具有保护作用。Objective To explore the effects of oxcarbazepine on apoptosis of hippocampus neuron in epileptic rats induced by pentyle- netetrazol（PTZ）. Methods Totally 84 cases of 4 - week - old rats were randomly divided into 4 groups ： the rats in group A were given 9g·L^-1saline（n = 18） ,the rats in group B were given oxcarbazepine（n = 18） ,group C and group D were epileptic groups （each group had 24 rats） , so the rats in group C were given 9 g·L^-1 saline after kindled, the rats in group D were given oxcarbazepine after kindled. The rats in group C and group D were given intraperitoneal injection of pentylenetetrazol （60 mg · kg^-1· d^-1）for 5 days, and rat＇s attack according to Racine classification was observed. The epileptic models were successfully constructed through 5 consecutive days of IV - V level attacks, each attack lasting 3 -5 minutes. On the 6th day, group A and group C were given 9g·L^-1 saline by intraperitoneal injection, group B and group D were given oxcarbazepine 80 mg·kg^-1·d^-1 by gavage. After continuous medication for 9,15 and 21 days, rats were killed and their brain were taken to make paraffin blocks. The morphological changes of apoptosis cells and number changes in hippocampus neuron of each group were observed by HE stains and immunohistochemical staining. Results HE staining：there was no obvious morphological difference between group A and group B. Group C showed vesical degeneration in cells, while the cell membrane invaginated. A large number of apoptotic cells could be found in group D, the cell layers increased. Bcl - 2 expression ： group A and group B had a small amount of Bcl - 2 expression in hippocampus of rats. At the time point of 9,15 and 21 days,before and after comparison between 2 groups there was no statistical difference. Compared with group A and group B, Bcl -2 positive cells in group C increased at the time point of 9,15 and 21 days. Compared with group A,group B and group C, expression of Bcl- 2 in group D was significantly increased at

关 键 词：癫痢 凋亡 奥卡西平 大鼠 

分 类 号：R96[医药卫生—药理学]