芝麻DNA高效提取及PAGE快速银染方法  

Methods for Efficient Extraction of Sesame Genomic DNA and Rapid Sliver Staining of PAGE

作　　者：吕海霞[1] 张艳欣[1] 王林海[1] 张晓燕[1] 张秀荣[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学重点开放实验室,武汉430062

出　　处：《中国农学通报》2010年第15期75-77,共3页Chinese Agricultural Science Bulletin

基　　金："十一五"国家科技支撑计划子课题"油料作物空间环境诱变育种关键技术研究与示范"(2008BAD97B04);国家自然科学基金"芝麻耐湿性QTLs定位及优异耐湿基因资源挖掘""芝麻茎点枯病抗性全基因组关联分析及有效分子标记研究"(30871552;30900905);中国农科院油料作物研究所所长基金课题"芝麻耐湿性遗传分化及其分子标记"(200708)

摘　　要：为了快速、低成本获得高质量的芝麻基因组DNA,在借鉴其他作物DNA常规提取方法基础上,简化了提取步骤,减少了试剂用量,DNA经琼脂糖电泳检测无拖尾,且OD260/OD280在1.8~2.0之间,表明质量、纯度均较高,摸索了一套芝麻DNA高效提取方法。在常规变性聚丙烯酰胺凝胶（PAGE）银染程序基础上进行改良,减少了固定环节和试剂用量,缩短了染色和显影时间等,优化建立了芝麻PAGE快速银染方法。In order to obtain high-quality sesame genomic DNA rapidly and economically for molecular experiments, an efficient method suitable for DNA extraction of sesame was explored, referred methods in other crops, the procedure was simplified and the cost was reduced, DNA were detected by agarose electrophoresis and no trailing phenomenon was found, their OD 260 /OD 280 values were all between 1.8-2.0, indicating that qualities of DNA were good and high purity. Afterwards, in order to further improve the efficiency of molecular marker experiments, a rapid and effective silver staining procedure for polyacrylamide gels was established, by improving the traditional silver staining methods, eliding the fixation, reducing cost and controlling the staining and imaging time.

关 键 词：芝麻 DNA高效提取 聚丙烯酰胺凝胶 快速银染 

分 类 号：S565.3[农业科学—作物学]