高钼对雏鸡肝脏和脾脏抗氧化功能的影响  

Effect of High Molybdenum on the Antioxidant′s Function of Liver and Spleen in Broilers

作　　者：肖杰[1] 杨帆[1] 崔恒敏[1] 彭西[1] 崔伟[1] 程安春[1] 陈涛[1] 柏才敏[1] 

机构地区：[1]四川农业大学动物医学院环境公害与动物疾病四川省高校重点实验室,雅安625014

出　　处：《畜牧兽医学报》2010年第7期883-890,共8页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："教育部长江学者和创新团队发展计划"创新团队项目(IRT0848);四川省重大培育项目;四川省科技厅基础应用研究项目;四川省教育厅重点项目;四川农大"双支"计划创新团队项目

摘　　要：本文旨在观察日粮高钼(Mo)对雏鸡肝脏和脾脏抗氧化功能的影响。300只1日龄Avian肉鸡健雏随机分为4组,分别喂以对照日粮(Mo 13 mg.kg-1)和高钼日粮(Mo 500 mg.kg-1,高钼Ⅰ组;Mo 1 000 mg.kg-1,高钼Ⅱ组;Mo 1 500 mg.kg-1,高钼Ⅲ组)42 d。与对照组比较,高钼Ⅱ组、Ⅲ组雏鸡肝脏和脾脏超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和黄嘌呤氧化酶(XOD)活性显著下降(P<0.01),丙二醛(MDA)含量显著升高(P<0.01);血清中相应酶活性和丙二醛含量变化与肝脏和脾脏中的一致。病理形态学观察,高钼组雏鸡肝细胞呈现不同程度的颗粒变性和空泡变性。流式细胞仪测定,高钼Ⅱ、Ⅲ组雏鸡脾脏细胞G0/G1期显著升高(P<0.01),S期、G2+M期和增殖指数(PI)显著降低(P<0.01)。结果表明,日粮钼含量1 000 mg.kg-1及其以上可引起肝脏和脾脏抗氧化功能降低,脾脏细胞增殖受阻。The experiment was conducted to examine the effect of dietary high molybdenum（Mo） on the antioxidant′s function of liver and spleen in broilers.300 one-day-old avian broilers were randomly divided into four groups,and fed on diets as follows： control diet（Mo 13 mg·kg^-1） and high molybdenum diets（Mo 500 mg·kg^-1,high molybdenum group Ⅰ;Mo 1 000 mg·kg^-1,high molybdenum groupⅡ;Mo 1 500 mg·kg^-1,high molybdenum group Ⅲ） for 6 weeks.The superoxide dismutase（SOD）,glutathion peroxidase（GSH-Px） and xanthine oxidase（XOD） activities were lower（P〈0.01） and the malondialdehyde（MDA）contents were higher（P〈0.01） in liver and spleen in high Mo groups Ⅱand Ⅲ than that in control group.Changes of the above mentioned enzymes activities and MDA contents in serum were consistent with those of liver and spleen.Pathologically,hepatocytes in high Mo groups showed granular degeneration and vacuolar degeneration.The statistical analyses by FCM indicated that the G0/G1 phase was increased（P〈0.01） and the G2＋M phase,S phase and the PI（Proliferating index） were decreased（P〈0.01） of spleen in high molybdenum groups Ⅱ and Ⅲ.The results showed that dietary molybdenum in excess of 1 000 mg·kg^-1 impaired antioxidant′s function in liver and spleen,inhibit the development of the spleen.

关 键 词：高钼 肝脏 脾脏 抗氧化功能 雏鸡 

分 类 号：S859.8[农业科学—临床兽医学]