外伤性视网膜脱离动物模型的建立及超微结构观察  

A new experimental model of traumatic retinal detachment in rabbits

作　　者：马汀[1,2] 王常观[1] 马志中[1] 徐航修[3] 姜彩辉[3] 

机构地区：[1]北京大学第三医院北京大学眼科中心,北京100083 [2]解放军401医院眼科,山东青岛266071 [3]解放军总医院眼科,北京100859

出　　处：《山东大学学报（医学版）》2010年第7期47-51,共5页Journal of Shandong University:Health Sciences

摘　　要：目的通过建立非外源性诱导物引发的实验性外伤性视网膜脱离动物模型,探讨外伤性视网膜脱离后视网膜的组织学改变。方法兔眼后上方角膜缘后2 mm平行切开巩膜,挤压出玻璃体,并向神经上皮层下注入眼用BSS,造成视网膜180°脱离,8-0可吸收线缝合伤口。以此制造外伤性视网膜脱离的动物模型。组织病理学光镜及电镜观察视网膜脱离模型视网膜的病理学变化。结果视网膜脱离后视网膜各层出现不同程度的退行性改变,以光感受器受损最为显著,神经元细胞成分减少、结构破坏,Müller细胞出现增生、肥大及胶质化等改变。结论此模型视网膜脱离发生率高,后期有PVR的发生,可用于视网膜脱离及引发增殖性玻璃体视网膜病变的研究。Objective To establish a new rabbit model of experimental traumatic retinal detachment caused by non-exogenous-inducer and to observe the corresponding histological changes in the retina.Methods A scleral incision was made 2 mm posterior and parallel to the limbus at the posterior and superior quadrant of the rabbit eye.The vitreous was squeezed out.BSS was injected into the sub-retinal space to produce a 180° retinal detachment.8-0 absorbable suture was used for the wound.Pathological changes were observed under a histopathological light microscope and electron microscope.Results Neural retina degeneration of various degrees was observed in all the layers of the retina after retinal detachment,with the most significant damages found in the photoreceptors.Decreased cellular components and damaged structure were observed in the neurons.Changes such as hyperplasia,hypertrophy and gliosis were found in the Müller cells.Conclusions This new animal model has the advantage of high incidence of retinal detachment and ideal development of proliferative vitreoretinopathy in the later stage,thus can be used for research on retinal detachment and proliferative vitreoretinopathy.

关 键 词：视网膜脱离 外伤 动物模型 

分 类 号：R774[医药卫生—眼科]