Cystamine对大鼠脑缺血再灌注后tTG表达的影响  

Effect of Cystamine on expression of tissue-type transglutaminase after global cerebral ischemia-reperfusion in rats

作　　者：孙丽[1] 李云[2] 朱美蓉[2] 亓蕾[2] 

机构地区：[1]山东大学医学院,济南250013 [2]山东大学附属济南市中心医院急诊科,济南250013

出　　处：《山东大学学报（医学版）》2010年第7期56-60,共5页Journal of Shandong University:Health Sciences

基　　金：山东省2009年度第一批济南留学人员创业计划资助项目(20090304)

摘　　要：目的研究胱氨酸(Cystamine)对全脑缺血再灌注损伤后大鼠海马组织型转谷氨酰胺酶(tTG)表达的影响。方法用四血管阻断法制作大鼠全脑缺血再灌注模型。将SD大鼠随机分为假手术组(n=8),全脑缺血组(n=48)及全脑缺血治疗组(n=48),全脑缺血组和全脑缺血治疗组按照缺血再灌注时间点的不同再将大鼠随机分为6h、12 h、1 d、3 d、5 d、7 d六个亚组,每个亚组8只。全脑缺血治疗组大鼠给予Cystamine腹腔注射(0.15 mg/g),全脑缺血组和假手术组大鼠给予生理盐水腹腔注射。TUNEL染色法观察细胞凋亡水平的变化,免疫组织化学方法分析再灌注后不同时间点海马CA1区tTG表达水平的变化。另取52只大鼠随机分为假手术组(n=4)、全脑缺血组(n=24)及全脑缺血治疗组(n=24),全脑缺血组和全脑缺血治疗组按照不同时间点每个亚组4只,免疫印迹方法测定tTG的表达。结果缺血组再灌注24 h后TUNEL阳性细胞明显增加,1、3、5、7 d亚组与假手术组差异有统计学意义(P<0.05);治疗组与缺血组相比,在1、3、5、7 d相应时间点亚组TUNEL阳性细胞数减少(P<0.05)。治疗组海马CA1区tTG平均阳性细胞数于全脑缺血后12 h开始下降,3、5、7 d亚组均明显低于缺血组(P<0.05)。治疗组海马tTG蛋白水平于缺血再灌注后1 d开始降低,3、5和7 d亚组显著低于缺血组(P<0.05)。结论 Cystamine可以降低大鼠全脑缺血再灌注后海马CA1区tTG的表达,从而对神经元细胞起到一定的保护作用。Objective To observe the effect of Cystamine on chronological expression of tissue-type transglutaminase after the hippocampal region of global cerebral ischemia-reperfusion injury in rats.Methods The experimental global cerebral ischemia-reperfusion injury model was established by the method of 4-vessel occlusion.Sprague-Dawley rats were randomly divided into the sham-operated group （ n = 8） ,the global cerebral ischemia group （ n = 48） and the glob- al cerebral ischemia treatment group（ n = 48） .The global cerebral ischemia group and the global cerebral ischemia treat- ment group were divided into six subsets of as 6,12 hour,1,3,5,7 day according to the time point with 8 rats each.Cystamine was injected intraperitoneally[0.15 mg /（ g·day） ]10 minutes after cerebral ischemia for the global cerebral ischemia treatment group.Saline was injected intraperitoneally for the global cerebral ischemia group and the sham-op- erated group.Neurons apoptosis was detected by TUNEL technology and expression of tTG was detected by immunohis- tochemistry（ SP） in the hippocampal CA1 region at different time points.The other 52 rats were randomly divided into the sham-operated group（ n = 4） ,the global cerebral ischemia group（ n = 24） and the global cerebral ischemia treatment group （ n = 24） .The global cerebral ischemia group and the global cerebral ischemia treatment group were divided into six subsets according to the time point with 4 rats each.Expression of tTG was detected by Western blotting.Results About 24h after ischemia-reperfusion injury ,the positive cells of TUNEL staining were significantly increased.Com- pared to the sham-operated group,the positive cells of TUNEL at the subset 1,3,5,7 day in the global cerebral ische- mia group were significantly increased（ P〈0.05） .The number of apoptotic cells in subset 1,3,5,7 day of the global cerebral ischemia treatment group were significantly less than that of the global cerebral ischemia group （ P〈0.05） .In the global cerebral

关 键 词：胱氨酸 脑缺血 再灌注损伤 组织型转谷氨酰胺酶 

分 类 号：R743.3[医药卫生—神经病学与精神病学]