SH2-B对结肠癌HT-29细胞增殖的影响  

SH2-B enhances proliferation of colon cancer HT-29 cells

作　　者：黄大毛[1] 李建国[2] 陈宇[1] 王巍巍[1] 吴尚辉[3] 段朝军[4] 唐发清[1,5] 

机构地区：[1]中南大学湘雅医院检验科,湖南长沙410008 [2]卫生部肝胆肠外科研究中心,湖南长沙410008 [3]中南大学湘雅医学院细胞中心,湖南长沙410078 [4]中南大学湘雅医院医学实验中心,湖南长沙410008 [5]暨南大学第三附属医院,广东珠海519000

出　　处：《中国普通外科杂志》2010年第7期768-773,共6页China Journal of General Surgery

基　　金：国家自然科学基金资助项目(30973400;30871189;30670990);湖南省科技厅计划项目(2009WK3060)

摘　　要：目的探讨SH2-B对结肠癌HT-29细胞周期演进和细胞增殖的影响。方法免疫荧光筛选SH2-B低表达结肠癌细胞(HT-29)。用LipofectAMINE2000将pcDNA3.1-SH2-B质粒转染至HT-29细胞(转染组),并设空载体转染组和未转染组作为对照。Western-blotting分析SH2-B转染后SH2-B的表达,MTT法分析SH2-B转染后HT-29细胞的增殖,流式细胞术分析SH2-B转染后HT-29细胞周期变化。结果将pcDNA3.1-SH2-B质粒转染至HT-29细胞后可获得SH2-B的有效表达。转染SH2-B后HT-29细胞增殖显著增强(P<0.05)。流式细胞仪检测结果显示,转染SH2-B组S期细胞显著高于未转染组和空载体组(P<0.05)。结论 SH2-B促进结肠癌HT-29细胞增殖和细胞周期演进。Objective To investigate the effect of SH2-B on proliferation and cell cycle of colon caner HT-29 cells.Methods Screening of the SH2-B low expression colon cancer cell line（HT-29） by immunofluorescence assay was done.pcDNA3.1-SH2-B was transfected into HT-29 cells using LipofecTAMINE 2000.After gene transfection,SH2-B expression was detected by Western-blotting,cell proliferation was measured by MTT assay,and cell cycle was analyzed by flow cytometer.Results Immunofluorescence assay results showed that SH2-B lowly expressed in HT-29 cells.after being transfected with pcDNA3.1-SH2-B,SH2-B expression dramatically increased in HT-29 cells,and HT-29 cells proliferation was significantly enhanced（P0.05）.Cell cycle analysis showed that S stage cells were significantly increased in the SH2-B transfected group（39.4±2.31） % compared with the blank control group（18.6±4.25）% and vector control group（11.1±2.39）%（P0.05）.Conclusions SH2-B may enhance colon cancer HT-29 cell proliferation and promote cell cycle.

关 键 词：结肠肿瘤 SH2-B HT-29 细胞增殖 

分 类 号：R735.3[医药卫生—肿瘤]