血管生成抑制素对胃癌细胞的影响  

Effect of angiostatin on human gastric adenocarcinoma cells

作　　者：乔洪旺[1] 康鹏程[1] 田墨涵[1] 张雅芳[1] 

机构地区：[1]哈尔滨医科大学解剖学教研室,黑龙江哈尔滨150081

出　　处：《解剖科学进展》2010年第4期315-318,共4页Progress of Anatomical Sciences

基　　金：哈尔滨市科技局留学归国人员基金(RC2006LX004011)

摘　　要：目的探讨血管生成抑制素对胃癌细胞增殖和游走的影响及对胃癌细胞分泌血管内皮生长因子A和C(VEGF-A和VEGF-C)的影响并分析其作用机制。方法采用MTT法和划线法观察0、0.5、1.0、1.5μg/ml浓度的血管生成抑制素对胃癌细胞增殖和游走的影响。免疫组化的方法检测0、0.5、1.0、1.5μg/ml浓度的血管生成抑制素对胃癌细胞内血管内皮生长因子(VEGF)-A和VEGF-C的影响。结果 MTT法显示不同浓度的血管生成抑制素对胃癌细胞增殖无明显影响(p>0.05),同样,划线法测得的胃癌细胞迁移距离同对照组相比无明显差异(p>0.05)。但免疫组化法结果显示,0.5、1.0、1.5μg/ml浓度的血管生成抑制素组胃癌细胞VEGF-A和VEGF-C的表达同对照组相比明显减少(p<0.05)。结论血管生成抑制素明显减少胃癌细胞内的VEGF-A和VEGF-C的分泌,但对胃癌细胞的增殖和游走无明显作用。Objective To study the effect and possible mechanism of angiostatin on the proliferation and migration of human gastric adenocarcinoma cells（HGC-27）. Method Scraping line method and MTT were used to observed the effect of 0、0.5、1.0、1.5μg/ml angiostatin on proliferation and migration of HGC-27, immunohistochemistry method was used to observe the effect of angiostatin on the expression of vascular endothelial growth factor（VEGF-A and VEGF-C） of HGC-27. Results MTT showed that the above concentrations of angiostatin did not play any role on the proliferation of HGC-27, at the same time, scraping line method showed that the above concentrations of angiostatin did not play any role on the migration of HGC-27. Immunohistochemistry showed that angiostatin downregulated the expression of VEGF-A and VEGF-C in HGC-27. Conclusion Angiostatin reduced the secretion of VEGF-A and VEGF-C in HGC-27, had no effect on the proliferation and migration of HGC-27.

关 键 词：血管生成抑制素 胃癌细胞 血管内皮生长因子-A 血管内皮生长因子-C 

分 类 号：R735.2[医药卫生—肿瘤]