脱脂程序对异体松质骨成骨能力影响的实验研究  

Influence of defatting procedure on osteogenicity of cancellous bone allograft

作　　者：朱加亮[1] 侯树勋[1] 衷鸿宾[1] 解英俊[1] 赵彦涛[1] 

机构地区：[1]解放军总医院第一附属医院骨科全军骨科研究所,北京100048

出　　处：《中国矫形外科杂志》2010年第14期1205-1208,共4页Orthopedic Journal of China

摘　　要：[目的]研究脱脂程序对同种异体松质骨移植后成骨能力的影响。[方法]取Lewis大鼠长骨干骺端作为移植骨块,随机分4组分别采用4种不同程序进行处理,检测处理后各组骨块内总脂肪和总蛋白含量。取Fisher 344大鼠作为骨移植受体,根据移植骨块不同随机分为4组,行股骨下干骺端骨缺损填充术。于取材前14d和4 d对受体大鼠行双荧光标记;术后8周时取出标本,不脱钙切片后行骨组织形态计量学分析。对各组的骨矿化速率(MAR)同相应骨块总脂肪含量行相关分析。[结果]超声清洗结合化学方法能较彻底去除骨块内脂肪成份。术后8周时取出标本不脱钙骨切片荧光显微镜下观察,均可见双色平行荧光带。影像分析系统测定骨矿化速率示D组最大,同其他各组比差异均有统计学意义(P<0.05)。骨矿化速率同总脂肪含量呈负相关。[结论]脱脂程序可提高异体松质骨移植后的成骨能力。超声清洗结合化学方法可较彻底清除骨内的脂肪成分和有效提高其移植后的成骨能力,可作为一种异体骨的有效处理方法。[Objective]To explore the influence of defatting procedure on osteogenicity of cancellous bone allograft.[Method]Thirty inbred Lewis rats were killed.Distal femoral metaphyses were resected as cancellous bone grafts.Grafts were randomly divided into four groups which were treated by four different procedures,respectively.Content of total lipid and total protein in grafts were measured.Twelve inbred Fisher344 rats were randomly divided into four groups according to different grouped grafts which were transplanted into femoral metaphysis bone defects of the recipients.Two different fluorochrome dyes were given subcutaneously to the recipients at 14^th and 4^th days before sacrifice respectively.All rats were killed at the 8^th postoperative week.The experimental distal femoral metaphyses were harvested and nondecalcified sections were prepared.Sections were observed with a fluorescent microscope and bone histomorphometries were studied.The correlation analysis was performed between mineral apposition rate（MAR） and the content of total lipid.[Result]Results of measurement showed content of total lipid in four grouped grafts were different and the difference between groups were statistically significant（P〈0.05）.At the 8^th postoperative week,flulargest microscopic observations showed double colored parallel lines were around margins of trabecula.MAR in group D was biggest and there was significant differences between group D and the other groups.The correlation analysis showed significant negative correlation between MAR and the content of total lipid（P〈0.05）.[Conclusion]Defatting procedures could increase osteogenicity of cancellous bone allograft.Procedure which combines ultrasonic cleaning with chemical method could defat the grafts more thoroughly and increase osteogenicity significantly.It might be an effective method to process allograft bone.

关 键 词：异体骨 移植 脱脂 成骨能力 组织形态计量学 

分 类 号：R687.3[医药卫生—骨科学]