丹参酮ⅡA对腹膜透析液诱导的大鼠腹膜组织学变化及TGF-β_1表达的影响  

Effects of tanshinone ⅡA on the morphology changes and the expression of transforming growth factor-beta 1 of peritoneal membranes in rats

作　　者：蒋春明[1] 陶娜娜[1] 孙琤[1] 张苗[1] 

机构地区：[1]南京大学医学院附属南京鼓楼医院肾内科,江苏南京210008

出　　处：《东南大学学报（医学版）》2010年第4期445-449,共5页Journal of Southeast University(Medical Science Edition)

基　　金：南京市医学科技发展重点项目(ZKX08026)

摘　　要：目的:了解丹参酮ⅡA对腹腔注射腹膜透析液(PDS)大鼠腹膜组织学变化以及转化生长因子-β1(TGF-β1)表达的影响。方法:50只SD雄性大鼠随机分为5组:对照组,每日用生理盐水腹腔注射20ml;PDS组,每日用4.25%的PDS腹腔注射20ml;丹参酮低、中、高浓度组,每日分别用含丹参酮ⅡA浓度为50、100、200mg·L-1的4.25%的PDS腹腔注射20ml。于实验第30天,取壁层腹膜行光镜检查,并用免疫组织化学的方法检测壁层腹膜TGF-β1的表达情况。结果:HE、Masson染色显示,在PDS干预下腹膜厚度显著增厚,胶原沉积显著增多。与PDS组比较,丹参酮各组有不同程度腹膜厚度减少,胶原沉积减少。腹膜间皮细胞在PDS干预下分泌TGF-β1较对照组显著增加,丹参酮各组TGF-β1分泌与PDS组比较均有显著下降。结论:丹参酮ⅡA具有抑制葡萄糖PDS导致的实验大鼠腹膜纤维化及TGF-β1表达增加的作用。Objective：To investigate the effects of tanshinoneⅡA on the alterations in morphology and the expression of transforming growth factor（TGF）-β1 of peritoneal membranes in a rat model of peritoneal dialysis.Methods：Fifty male Sprague-Dawley（SD） rats were randomly divided into five groups（n =10 in each group）.Intraperitoneal injection of 0.9% saline,standard lactate-buffered 4.25% glucose-based PDS,and standard lactate-buffered 4.25% glucose-based PDS with various concentrations of tanshinone ⅡA（50,100,200 mg·L-1） were performed for rats in each group once daily with an instillation volume of 20 ml per injection for 30 days.Then,histological analyses,including hematoxylin and eosin（HE） staining and Masson staining,were carried out in parietal peritoneum.Immunohistochemistry were used to analyze the expression of TGF-β1.Results：As compared with control group,chronic high-glucose-based PDS exposure resulted in increased submesothelial compact zone thickness and accumulation of submesothelial matrix,as well as enhanced expression of TGF-β1 in parietal peritoneum.The peritoneal changes in-duced by PDS were significantly ameliorated by tanshinone ⅡA.Conclusion：Long-term exposure to high glucosebased PDS results in damages in peritoneal membranes and promotes the fibrosing process.Tanshinone ⅡA may be helpful in slowing the PDS-induced deterioration of peritoneal membrane.

关 键 词：丹参酮ⅡA 腹膜纤维化 转化生长因子-Β1 腹膜透析 大鼠 

分 类 号：R572.2[医药卫生—消化系统] R453[医药卫生—内科学]