人类疱疹病毒-5～8型与口腔扁平苔藓发病间的关系  

Relationship between human herpes virus-5 to 8 and oral lichen planus

作　　者：顾杨[1] 李晶泉 李铭汉[1] 宫良文[1] 徐昊 张虹[1] 李文颜[1] 陈政翰 

机构地区：[1]大连医科大学口腔医学院口腔内科学教研室,辽宁大连116027 [2]宝生物工程大连有限公司,辽宁大连116600

出　　处：《国际口腔医学杂志》2010年第4期386-391,共6页International Journal of Stomatology

基　　金：辽宁省科委和教委联合基金资助项目(辽财企字2005-696)

摘　　要：目的探索人类疱疹病毒(HHV)-5～8型与口腔扁平苔藓(OLP)发病之间的关系。方法选用实时定量聚合酶链反应检测方法,分别采集36例OLP患者的外周血白细胞、血浆、唾液以及病损区脱落细胞、非病损区脱落细胞和活检组织作为研究对象,另外选取43例健康人相同部位组织作为对照组。结果 HHV-5在OLP组中的阳性样本检出率依次为白细胞50.00%(8/16)、唾液25.00%(4/16)、活检组织80.00%(4/5)、病损区脱落细胞50.00%(8/16)、非病损区脱落细胞18.75%(3/16),健康对照组则依次为白细胞30.00%(3/10)、唾液20.00%(2/10);HHV-6在OLP组中的阳性样本检出率依次为白细胞18.75%(3/16)、活检组织40.00%(2/5)、病损区脱落细胞12.50%(2/16),健康对照组仅唾液10.00%(1/10);HHV-7在OLP组中的阳性样本检出率仅有唾液30.00%(6/20),健康对照组也仅有唾液15.00%(3/20);HHV-8在所有样本中未检出。结论 HHV-5与OLP的发病有关联(χ2=6.8290,P<0.05,Pearson列联系数0.2080),HHV-5检出的病毒拷贝数在试验组内的白细胞列与病损脱落细胞列之间差异有统计学意义(P<0.01)。但HHV-6和7与OLP的发病关系不能确定。Objective The aim of this study is to survey the potential relationship between oral lichen planus （OLP） and human herpesvirus（HHV）-5 to 8. Methods Seriously selected 36 OLP patients and 43 healthy controls, and meanwhile we collected their 6 kinds of samples including white blood cell（WBC）, serum, saliva, exfoliated cel（lun-lesion and lesion sections）, and biopsy samples. The real-time polymerase chain reaction technology was employed to explore the DNA copies of HHV-5 to 8. Results The HHV-5 positive rates in OLP samples are, WBC 50.00%（8/16）, saliva 25.00%（4/16）, biopsies 80.00%（4/5）, exfoliated cells from un -lesion sections 18.75%（3/16）and lesion sections 50.00%（8/16） respectively. The counterpart in healthy controls are, saliva 20.00%（2/10） and WBC 30.00%（3/10）, respectively. The HHV-6 positive rates in OLP samples are WBC 18.75%（3/16）, biopsies 40.00%（2/5） and exfoliated cells from un-lesion sections 12.50%（2/16） respectively. The counterpart in healthy controls is only in saliva 10.00%（1/10）. The only HHV-7 positive rate is in OLP saliva 30.00%（6/20）. The counterpart in healthy controls is also in saliva 15.00%（3/20）. However, the copy of HHV-8 could not be explored in all samples. Conclusion The Chi-square test（x2=6.829 0, P0.05） and the value of Pearson contingency coefficient（0.208 0） showed that HHV-5 was significantly closely related with patients with OLP, but HHV-6 and 7 were not. The number of HHV-5 DNA copy has a significant different between WBC and exfoliated cells from un-lesion sections in OLP group（P=0.001）.

关 键 词：口腔扁平苔藓 人类疱疹病毒 实时定量聚合酶链反应 

分 类 号：R781.5[医药卫生—口腔医学]