低氧下丹参酮ⅡA对人胃癌SGC7901细胞HIF—1α与c—Met表达的影响  

Effects of Tan Ⅱ A on expression of HIF- 1α and c- Met in gastric cancer SGC7901 cells under hypoxia

作　　者：冯玉光[1] 宗绪山[1] 邢国辉[1] 朱芸[1] 吴美英[1] 

机构地区：[1]潍坊医学院附属医院消化内科,261042

出　　处：《国际肿瘤学杂志》2010年第5期394-396,共3页Journal of International Oncology

基　　金：山东省中医药科技发展计划项目（2007-156）

摘　　要：目的观察低氧下丹参酮ⅡA（Tan ⅡA）对人胃癌SGC7901细胞c—Met蛋白表达的影响及其与低氧诱导因子-1α（HIF-1α）表达的关系。方法用氯化钴（CoCl2）创建低氧模型，设常氧对照组、低氧对照组和低氧加不同浓度Tan ⅡA组。分别取0．5、2．0、10．0mg／L Tan ⅡA干预低氧胃癌细胞48h后，免疫细胞化学二步法检测HIF-1α和c-Met蛋白表达的变化。结果免疫细胞化学法显示，TanⅡA呈剂量依赖性地抑制低氧诱导的HIF-1α和c-Met蛋白表达，且二者呈高度正相关（n=4，r=0．996，P〈0．01）。结论低氧条件下，TanⅡA可能通过抑制HIF-1α的表达进而抑制c-Met蛋白的表达，提示TanⅡA可能对防治低氧导致的肿瘤侵袭转移具有萤要作用。Objective To study the effects of Tan Ⅱ A on the expression of c- Met and HIF- 1α in gastrie cancer SGC7901 cells under hypoxia and the correlation between c-Met and HIF-1α expression. Methods The model of hypoxia was established by CoCl2. There were three groups ： normal control group, hypoxia control group, and hypoxia combined with different concentrations of Tan Ⅱ A group. After Tan Ⅱ A was added to the media with 0. 5, 2. 0 and 10. 0 mg/L respectively for 48 h under hypoxia, the expression of HIF-1α and c -Met protein was detected by immunoeytochemieal staining. Results Immunocytochemical staining revealed that the expression of HIF- 1α and c- Met protein was inhibited by Tan Ⅱ A in a close- dependent manner under hypoxia. High positive correlation was found between the expression of HIF- 1α and c- Met （ n = 4, r = 0. 996, P 〈 0. 01 ）. Conclusion Tan Ⅱ A might inhibit the expression of c - Met protein via down- regulation of HIF- 1α protein expression under hypoxia, suggesting that Tan Ⅱ A might play an important role in fighting against hypoxia-stimulated invasion and metastasis of tumor.

关 键 词：胃肿瘤 丹参酮ⅡA 缺氧 缺氧诱导因子-1 

分 类 号：R735.2[医药卫生—肿瘤]