姜黄素对大鼠肝星状细胞表达受体型蛋白酪氨酸磷酸酶的影响的实验研究  

Effect of curcumin on the progress of liver fibrosis

作　　者：樊海宁[1] 王聪[1] 任利[1] 阳丹才让 周瀛[1] 侯立朝[1] 邓勇[1] 

机构地区：[1]青海大学附属医院肝胆胰外科,青海西宁810001

出　　处：《现代生物医学进展》2010年第13期2404-2408,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金资助项目(30960374)

摘　　要：目的:本研究通过观察受体型蛋白酪氨酸磷酸酶(PTPRJ)在大鼠肝星状细胞中的表达及姜黄素对其的影响,探讨以PTPRJ为靶点,姜黄素对抗肝纤维化的分子机制。方法:四氯化碳(CCL4)皮下注射诱导大鼠肝纤维化模型,并采用Percoll梯度离心法分离培养原代大鼠肝星状细胞,采用western-blot、RT-PCR法分别测定PTPRJ、其mRNA在肝组织及肝星状细胞的表达变化及姜黄素对其表达的影响。结果:western-blot、RT-PCR检测结果显示:肝组织和肝星状细胞中PTPRJ表达呈阳性,并随着肝纤维化进展,其表达逐渐降低;姜黄素作用后,肝组织和肝星状细胞PTPRJ表达明显增多。结论:姜黄素可显著增强肝星状细胞PTPRJ的表达,从而改善或逆转肝纤维化进展。Objective：Protein tyrosine phosphatase,receptor-type J（PTPRJ）is expressed in a wide variety of tissues including liver.The aim of this study was to investigate the expression of PTPRJ in rat hepatic stellate cell（HSC）and the influence of curcumin on this.Methods：In CCL4,intoxicated rat livers and HSCs,PTPRJ expression was measured by western-blot and RT-PCR.And then we observed the influence of cmrcumin on PTPRJ＇ expression.Results：In CCl4-intoxicated rat livers,PTPRJ expression was decreased with time,as the IeveI of hepatic fibrosis increased.But PTPRJ expression observed by western-blot and RT-PCR was gradually stronger after curcumin treated.Conclusion：PTPRJ was expressed in rat HSCs.It may have negative effect on HSC activation,proliferation and liver fibrosis.And curcumin treated can be stronger its expression.

关 键 词：受体型蛋白酪氨酸磷酸酶 肝纤维化 肝星状细胞 姜黄素 

分 类 号：Q95-3[生物学—动物学] R575[医药卫生—消化系统]