丹参酮ⅡA联合5-FU对人胃癌SGC7901细胞增殖和凋亡与突变型p53蛋白表达的研究  

Effect of Tan ⅡA combined with 5-FU on proliferation and apoptosis of SGC7901 human gastric cancer cells and its relationship with expression of mutant p53

作　　者：冯玉光[1] 邢国辉[1] 宗绪山[1] 朱芸[1] 吴美英[1] 

机构地区：[1]潍坊医学院附属医院消化内科,山东潍坊261042

出　　处：《中华肿瘤防治杂志》2010年第11期831-834,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：山东省中医管理局中医药科技发展计划项目(2007-156)

摘　　要：目的:观察丹参酮ⅡA(TanⅡA)联合5-氟尿嘧啶(5-FU)对人胃癌SGC7901细胞的增殖抑制和诱导凋亡作用,及其与突变型p53蛋白表达变化的关系。方法:不同浓度的TanⅡA单独及联合应用10.0 mg/L的5-FU作用于SGC7901细胞24、48和72 h,MTT法检测细胞活力,HO-ECHST染色法检测细胞凋亡;不同浓度的TanⅡA单独及联合10.0 mg/L的5-FU作用于SGC-7901细胞48 h后,免疫细胞化学法检测突变型p53蛋白的表达。结果:实验所选各种浓度TanⅡA均能抑制SGC7901细胞的增殖并诱导其凋亡,且呈时间和剂量依赖性(P值均<0.01),10.0 mg/L TanⅡA作用细胞72 h后,其抑制率及凋亡率分别达47.17%和45.63%。与单用5-FU相比,联合应用TanⅡA细胞增殖抑制率和凋亡率显著增加(P均<0.01),10.0 mg/L TanⅡA联合5-FU作用细胞72 h后,其抑制率及凋亡率分别为65.51%和57.28%。不同浓度的TanⅡA单独及联合应用5-FU作用48 h后,突变型p53表达呈剂量依赖性降低(P均<0.01)。结论:TanⅡA可显著增强5-FU对SGC7901细胞的增殖抑制和诱导凋亡作用,该效应可能与其抑制突变型p53蛋白的表达有关。OBJECTIVE： To study the effect of Tan ⅡA combined with 5-fluorouracil（5-FU） on the proliferation and apoptosis of the SGC7901 human gastric cancer cells and its relationship with the expression of mutant p53（mt p53）.METHODS： SGC7901 cells were treated by different concentrations of Tan ⅡA with or without 10.0mg/L 5-FU for 24,48 and 72h.The cells proliferative vitality was detected by MTT assay,and the apoptosis of SGC7901 cells was detected by HOECHST stain.After Tan ⅡA at different concentrations with or without 10.0 mg/L 5-FU was added to the media respectively for 48 h,the expression of mt p53 protein was detected by immunocytochemical staining.RESULTS： Tan ⅡA at different concentrations inhibited the proliferation and induced the apoptosis of SGC7901 cancer cells in a dose-and time-dependent manner（both P〈0.01）,the inhibition rate and apoptosis rate reached 47.17% and 45.63% after treatment with 10.0 mg/L Tan ⅡA for 72 h.Compared with using 5-FU alone,Tan ⅡA combined with 5-FU inhibited the proliferation of cells and increased the apoptosis rates more significantly（both P〈0.01）,the inhibition rate and apoptosis rate reached 65.51%,57.28%,respectively after the treatment with 10mg/L Tan ⅡA combined with 5-FU for 72 h.After SGC7901 cells were treated by different concentrations of Tan ⅡA with or without 5-FU for 48h,mt p53 protein expression was significantly down-regulated in a dose-dependent manner（both P〈0.01）.CONCLUSION： Tan ⅡA can significantly enhance the growth inhibition and apoptosis inducation of 5-FU on SGC7901 cells,which may be related to the down regulation of mt p53 protein expression.

关 键 词：胃肿瘤/病理学 丹参酮 氟尿嘧啶 细胞增殖 细胞凋亡 基因 p53 

分 类 号：R735.2[医药卫生—肿瘤]