Livin靶向siRNA对大肠癌细胞凋亡的诱导作用  

Apoptosis induction effect of siRNA recombinant expression vector targeting Livin gene in human colon cancer cells

作　　者：蔡明[1] 王国斌[1] 陶凯雄[1] 韩高雄[1] 蔡昌学[2] 

机构地区：[1]华中科技大学同济医学院附属协和医院普外科,湖北武汉430022 [2]华中科技大学同济医学院病原生物系微生物学教研室,湖北武汉430030

出　　处：《中华肿瘤防治杂志》2010年第12期890-893,共4页Chinese Journal of Cancer Prevention and Treatment

基　　金：国家863高技术研究发展计划资助项目(2001AA218051);湖北省科技攻关计划资助项目(2005A304B09)

摘　　要：目的:构建Livin靶向小干扰RNA(siRNA)重组表达载体,观察其诱导大肠癌细胞的凋亡效应。方法:构建Livin靶向siRNA重组表达载体并转染结肠癌细胞,RT-PCR和蛋白质印迹法检测Livin的表达,MTT法检测siRNA对细胞增殖的抑制作用,流式细胞仪检测细胞的凋亡效应。结果:经酶切鉴定和测序结果证实,Livin靶向siRNA重组表达载体构建成功,它对大肠癌细胞LivinmRNA和蛋白的表达抑制率分别为30.18%和28.88%,P<0.05,肿瘤细胞的生长受到明显抑制,细胞凋亡率为(13.36±1.45)%,P<0.05。结论:Livin靶向siR-NA重组表达载体构建成功,能有效抑制Livin基因表达并能显著诱导肿瘤细胞凋亡。OBJECTIVE：To construct siRNA recombinant expression vector targeting Livin gene and observe the apoptosis induction effect of it in human colon cancer cells.METHODS：siRNA recombinant expression vector targeting Livin gene was constructed and transfected into human colon cancer cells.The effect of siRNA recombinant expression vector was detected by RT-PCR,Western blot,MTT reduction assay and flow cytometry.RESULTS：It was confirmed by restriction endonuclease and sequence analysis that siRNA recombinant expression vector targeting Livin gene was constructed successfully.The inhibition ratios of Livin siRNA at mRNA and protein levels were 30.18% and 28.88% respectively （P〈0.05）.The growth of cancer cells was inhibited significantly and the apoptotic ratio was （13.36±1.45）% （P〈0.05）.CONCLUSIONS：The siRNA recombinant expression vector targeting Livin gene has been constructed successfully.It can not only inhibite the expression of Livin gene but also induce apoptosis in human colon cancer cells remarkably.

关 键 词：结直肠肿瘤 LIVIN RNA干扰 基因治疗 

分 类 号：R735.34[医药卫生—肿瘤]