外源性FHIT基因对人乳腺癌MDA-MB-436细胞恶性表型的影响  

Effects of exogenous FHIT gene on malignant phenotype of human breast cancer cell line MDA-MB-436

作　　者：王旭光[1] 陈根殷[2] 杨展[1] 何惠娟[1] 吴平[1] 吴英健[1] 

机构地区：[1]广东医学院附属医院中心实验室,广东湛江524001 [2]广东医学院附属医院麻醉科,广东湛江524001

出　　处：《中华肿瘤防治杂志》2010年第13期979-981,共3页Chinese Journal of Cancer Prevention and Treatment

基　　金：广东省卫生厅科研项目(B2007143)

摘　　要：目的:研究外源性脆性组氨酸三联体(FHIT)基因的表达对乳腺癌MDA-MB-436细胞增殖的影响并探讨其机制。方法:通过脂质体将外源性FHIT基因的真核表达质粒PEGFP-FHIT和空载体分别转染FHIT表达缺失的乳腺癌细胞MDA-MB-436。MTT法分析细胞增殖,流式细胞术观察细胞的凋亡,蛋白质印迹法检测外源性FHIT基因及凋亡相关基因Caspase-3、-8、-9的蛋白表达。结果:PEGFP-FHIT组细胞的生长抑制率和凋亡率明显高于空载体组和对照组,PEGFP-FHIT组细胞Caspase-3、-9活性片段表达上调。结论:外源性FHIT基因表达能抑制MDA-MB-436细胞增殖,其机制可能是通过激活Caspase途径从而诱导细胞凋亡。OBJECTIVE： To investigate the effects of the FHIT gene on the malignant growth of breast cancer cell line MDA-MB-436 and its mechanism of cancer inhibition.METHODSD： Mammaliam expression vector PEGFP-FHIT was transfected into the breast cancer cell line MDA-MB-436 by liprofectamine.The effect of FHIT on the growth of MDA-MB-436 was examined by MTT and flow cytometry.The expressions of exogenous FHIT protein and Caspase-3,-8,-9 were detected by Western blot.RESULTS： The growth inhibitory rate and apoptosis rate of the cells in PEGFP-FHIT group were significantly higher than those in the PEGFP group and control group.The expressions of caspase-3,-9 active proteins were significant increased in the PEGFP-FHIT group.CONCLUSION：The expressions of exogenous FHIT gene can obviously suppress the proliferation and induce apoptosis by the activation of the caspase pathway in MDA-MB-436 cells.

关 键 词：脆性组氨酸三联体基因 乳腺肿瘤 基因转染 细胞凋亡 

分 类 号：R737.9[医药卫生—肿瘤]