人类骨髓成纤维样基质细胞对多发性骨髓瘤细胞迁移和归巢特性的影响  

Effects of human bone marrow fibroblastoid stromal cell line HFCL on the biological characteristics of migrating and homing in multiple myeloma cell line RPMI8226

作　　者：葛繁梅[1] 高延慧[1] 白庆咸[2] 

机构地区：[1]延安大学医学院附属医院血液科,716000 [2]第四军医大学西京医院血液科

出　　处：《白血病．淋巴瘤》2010年第7期418-420,共3页Journal of Leukemia & Lymphoma

摘　　要：目的 观察人类骨髓成纤维样细胞系HFCL对多发性骨髓瘤细胞RPMI8226增殖、迁移和归巢的影响.方法 采用体外细胞培养,建立RPMI8226细胞和HFCL细胞共培养体系,锥虫蓝拒染法测定生长曲线;流式细胞术（FCM）检测细胞周期和黏附分子CD49d的变化;RT-PCR检测CXCR4基因的表达情况.结果 HFCL细胞抑制RPMI8226细胞生长,且与HFCL细胞直接接触组的抑制作用大于非直接接触组（Transwell）组.RPMI8226细胞与HFCL细胞共培养后,直接接触组G1期细胞增高,S期细胞减少：HFCL细胞下调RPMI8226细胞的CXCR4和CD49d 的表达,单独培养组明显高于直接培养组;Transwell组无明显变化.结论 人类骨髓成纤维样细胞HFCL能抑制多发性骨髓瘤细胞RPMI8226的增殖,抑制CXCR4和CD49d的表达,影响骨髓瘤细胞的迁移和归巢.Objective To investigate the effects of human bone marrow fibroblastoid stromal cell lines HFCL on the proliferation .migrating and homing of multiple myeloma cell lines RPM18226. Methods The co-culture system of RPMI8226 and HFCL was established through cell culture in vitro, growth curves were determined by trypan blue exclusion, cell cycle and expression of adhension molecule CD49d were detected by flow cytometry, and expression of CXCR4 gene was examined by RT-PCR. Results The proliferation of RPMI8226 cells in direct contact with HFCL cells group was inhibited strongerly than that in transwell group. The percentage of G1 phase cells of RPMI8226 cells in direct contact with HFCL group was higher than that of RPM 18226 in transwell group, while the percentage of S phase cells was lower. The expressions of CD49d and CXCR4 in RPMI8226 cells were down-regulated by HFCL cells, and that in transwell group was higher than that in direct contact with HFCL cells group. Conclusion Human bone marrow fibroblastoidss tromal cell HFCL can inhibit the proliferation and the expressions of CD49d and CXCR4 of multiple myeloma cell line RPMI8226, and can prevent multiple myeloma cells from migrating and homing.

关 键 词：多发性骨髓瘤 骨髓 成纤维样细胞 受体 CXCR4 CD49D 整合素Α4 

分 类 号：R733.3[医药卫生—肿瘤]