吡拉西坦氯化钠对大鼠创伤脑组织内AQP9表达的影响  

Effect of piracetam on expression of AQP9 after ischemic brain edema in rat

作　　者：王东[1] 张建军[1] 杨忠旭[1] 

机构地区：[1]天津市第四中心医院神经外科,天津300140

出　　处：《中国现代医学杂志》2010年第13期1948-1951,1956,共5页China Journal of Modern Medicine

摘　　要：目的探讨吡拉西坦氯化钠注射液对大鼠外伤性脑水肿脑组织中AQP9基因表达和蛋白合成的影响。方法健康SD大鼠144只,随机分为:对照组、手术组、吡拉西坦氯化钠注射液组,采用液压颅脑损伤仪,给予2.5～3.0atm液压冲击力,制成重型液压颅脑损伤模型,分别在6h、1d、3d和5d4个时间点将大鼠麻醉后断头取脑,采用干湿比重法测脑组织含水量,行HE染色,并用RT-PCR、WesternBlot检测脑组织中AQP9基因表达和蛋白合成的变化。结果与对照组相比,手术组、吡拉西坦氯化钠注射液组均从6h开始出现脑水肿,3d时达到高峰,且吡拉西坦氯化钠注射液组脑水肿低于手术组;同样AQP9及其mRNA的表达在手术后6h后开始升高,3d后达到高峰,且手术组较对照组相和吡拉西坦氯化钠注射液组明显增高(P<0.05)。结论 AQP9与脑水肿形成有关,吡拉西坦氯化钠注射液可以降低脑组织内AQP9的基因表达和蛋白合成从而减轻脑水肿。[ Objective ] To study effects of piraeetam on the expression of AQP9 mRNA and AQP9 after traumatic cerebral edema in rats. [Methods] 144 normal SD rats were randomized to control group, surgery group and piraeetam group.AQP9 mRNA and AQP9 expression were determined by RT-PCR and Western Blot at 6 h, 1 d.3 d,5 d. The models of severe TBI were made in the rats by giving 2.5-3.0 atm power of hydraulic impact. At each time point, the water content in the brain tissues was measured as wet-to-dry weight ratio. The pathology was checked at each time too. [Result] Compared with the control group, cerebral edema appeared in surgery group and piracetam group 4 h after operations, it reached peak at 3d and cerebral edema of piraeetam group was lower than surgery group. The AQP9 mRNA and AQP9 expression of surgery group were obviously higher than those in the control group and piracetam group （P〈0.05）,＇and they increased at 6 h, reached peak at 3 d. [Conclusion] AQP9 may be involved in the formation of brain edema, piracetam, can decrease the expression of AQPgmRNA and AQP9 and relieve brain edema.

关 键 词：水通道蛋白-9 大鼠 创伤性脑水肿 吡拉西坦 

分 类 号：R-332[医药卫生]