TGF-β1 shRNA对涎腺人黏液表皮样癌裸鼠颌下腺移植瘤的抑瘤作用及Ki-67、VEGF蛋白表达的影响  

Inhibitory Effect of TGF-β1 shRNA on Human Salivary Gland Mucoepidermoid Carcinoma in Nude Mice Submandibular Gland and Effect of Ki-67 and VEGF Expression

作　　者：王静[1] 陈健[2] 马铭[1] 赵媛[1] 吴军正 

机构地区：[1]兰州大学口腔医学院,730000 [2]兰州大学第一医院 [3]第四军医大学口腔医院

出　　处：《肿瘤防治研究》2010年第7期735-738,共4页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金资助项目(30371551);兰州大学医学基金资助项目(LZUYX200707);兰州大学中央高校基本科研费自由探索资助项目(面上项目)(lzujbky-2009-96)

摘　　要：目的研究TGF-β1 shRNA对人涎腺黏液表皮样癌(Ms)细胞裸鼠颌下腺原位移植瘤成瘤能力以及对Ki-67和VEGF蛋白表达的影响。方法将Ms细胞、稳定转染空载体的Ms细胞、稳定转染TGF-β1 shRNA的Ms细胞分别接种至裸鼠颌下腺,观察各组细胞在裸鼠体内的成瘤率,测量肿瘤体积、瘤质量并绘制肿瘤生长曲线;免疫荧光法和免疫组织化学法检测肿瘤组织中Ki-67和VEGF蛋白的表达。结果与未转染组、转染空载体组相比,转染TGF-β1 shRNA组裸鼠颌下腺原位移植瘤生长缓慢,瘤体体积和瘤质量显著降低(P<0.01),瘤细胞中Ki-67和VEGF蛋白表达明显降低。结论 TGF-β1 shRNA能显著抑制Ms细胞在体内的生长能力,其机制可能与下调细胞内Ki-67和VEGF水平有关。Objective To study inhibitory effect of TGF-β1 shRNA on human salivary gland mucoepidermoid carcinoma in nude mice submandibular gland and the effect of Ki-67 and VEGF expression.Methods Nude mice submandibular gland were transplanted with Ms cells,Ms cells stably transfected with empty vectors,Ms cells stably transfected with TGF-β1 shRNA,respectively.The tumor volume and weight were measured and observed by growth curve.Immunohistochemistry and immunoflurescence were used to detect the level of Ki-67 and VEGF.Results Compared with Ms cells and Ms transfected with empty vectors,Ms cells stably transfected with TGF-β1 shRNA showed slow growth in tumor volume and weight （P〈0.01）,as well as down-regulated the expressions of Ki-67 and VEGF.Conclusion TGF-β1 shRNA could inhibit the tumor growth of Ms cells,the mechanism of which might be related with down-regulation of Ki-67 and VEGF.

关 键 词：TGF-Β1 SHRNA 黏液表皮样癌 KI-67 VEGF 

分 类 号：R782.4[医药卫生—口腔医学] R739.8[医药卫生—临床医学]