马钱子碱对白血病K562/A02细胞多药耐药性的逆转作用  

A Study on Vauqueline in Reversal of Multidrug Resistant in K562/A02 Cells

作　　者：梁虹[1] 茆俊卿 张育[1] 高娜[1] 沈维干[1] 顾健[1] 

机构地区：[1]江苏扬州大学医学院,225001 [2]江苏扬州市中医院

出　　处：《肿瘤防治研究》2010年第7期739-743,共5页Cancer Research on Prevention and Treatment

基　　金：江苏省中医药管理局资助课题(H05110)

摘　　要：目的研究马钱子碱(vauqueline)对人白血病K562/A02细胞多药耐药性的逆转作用。方法采用噻唑蓝(MTT)法检测马钱子碱的细胞毒作用;采用半定量逆转录聚合酶链反应(RT-PCR)和免疫印迹(Western blot)分别检测非细胞毒浓度(IC10)的马钱子碱对K562/A02细胞MDR1(multidrug resistance gene1)、多药耐药相关蛋白(multidrug resistance-associated protein,MRP)、拓扑异构酶Ⅱ(topoisomeraseⅡ,TopoⅡ)、谷胱苷肽-S-转移酶(glutathione s-transferase,GST-π)mR-NA及其蛋白表达的影响。结果非细胞毒浓度(IC10)的马钱子碱作用后,K562/A02细胞中MDR1mRNA及P-gp表达降低(P<0.01)。而MRP、TopoⅡ、GST-πmRNA及其蛋白的表达无明显变化(P>0.05),同时马钱子碱能增加化疗药物在白血病细胞内的积累。结论马钱子碱能部分逆转K562/A02细胞的耐药性,其作用机制可能与下调K562/A02细胞MDR1mRNA的表达,导致细胞膜上P-gp的表达量减少,化疗药物从细胞内溢出减少有关。Objective The purpose of this study is to investigate the reversal effects of Vauqueline on human leukemic multidrug resistance（MDR） in K562/A02 cell line MDR1（multidrug resistance 1）,MRP（multidrug resistance-associated protein）,TopoⅡ（topoisomeraseⅡ）,GST-π （glutathione-s-transferase）genes and their proteins.Methods The MTT assay was used to analyze the cytotoxic effect of vauqueline. The expression of MDR1,MRP,TopoⅡ,GST-π and their proteins in leukemia K562/A02 cells were respectively detected by semi-quantitative reverse transcription polymerase chain reaction （RT-PCR） and Western Blot.Results Semi-quantitative RT-PCR showed that mRNA transcription of MDR1 gene was reduced significantly（P〈0.01）; and the other genes were not conspicuously changed （P〉0.05）.Western blot showed that the expression of P-gp was decreased （P〈0.01 ）; the other proteins were not conspicuously changed（P〉0.05）.Vauqueline could increase the accumulation of medicine in K562/A02 cells.Conclusion Vauqueline could partly reverse the multidrug resistance of K562/A02 cells.Decreasing of MDR1 gene and p-glycoprotein expression and increase the accumulation of medicine in cells may be the MDR reversal mechanisms in K562/A02 cells.

关 键 词：马钱子碱 K562/A02细胞 多药耐药 逆转 

分 类 号：R730.53[医药卫生—肿瘤] R733.7[医药卫生—临床医学]