乳腺浸润性导管癌中AKT和VEGF-C的表达及其与淋巴道转移的相关性  

Expression of AKT and VEGF-C in Breast Invasive Ductal Carcinoma and Their Correlations with Lymphatic Metastasis

作　　者：李小芳[1] 姜汉国[1] 李飞虹[1] 蔡琼珍[1] 

机构地区：[1]广东医学院附属医院病理科,广东湛江524001

出　　处：《肿瘤防治研究》2010年第7期781-785,794,共6页Cancer Research on Prevention and Treatment

摘　　要：目的探讨AKT、p-AKT、AKT2及VEGF-C表达与乳腺浸润性导管癌淋巴结转移的关系,以及乳腺浸润性导管癌中AKT、p-AKT和AKT2与VEGF-C表达的关系。方法采用RT-PCR法检测17例乳腺浸润性导管癌新鲜组织中AKT2和VEGF-CmRNA的表达;用免疫组织化学SP法检测74例乳腺浸润性导管癌石蜡标本中AKT、p-AKT、AKT2和VEGF-C蛋白的表达,用Western blot法检测17例乳腺浸润性导管癌新鲜标本中AKT、p-AKT、AKT2和VEGF-C蛋白的表达。结果 AKT2 mRNA在淋巴结转移组中的表达高于无淋巴结转移组(U=14.000,P=0.043),VEGF-C mRNA在两组乳腺浸润性导管癌组织中表达水平差异无统计学意义(U=29.000,P=0.601);AKT2和VEGF-C蛋白在有淋巴结转移组中的表达均高于无淋巴结转移组(P<0.05),而AKT及p-AKT的表达在两组间差异无统计学意义(P>0.05);p-AKT、AKT2及VEGF-C蛋白在淋巴结转移组中表达均高于无淋巴结转移组;在乳腺浸润性导管癌中VEGF-C与AKT,VEGF-C与p-AKT的表达均呈显著正相关(r分别为0.283和0.328,P值分别为0.015和0.004),VEGF-C与AKT2的表达无相关性(r=0.072,P=0.543)。结论 AKT2及VEGF-C与乳腺浸润性导管癌淋巴结转移密切相关,可作为乳腺浸润性导管癌淋巴结转移的预测因子。Objective To discuss the relationship between AKT,p-AKT,AKT2,VEGF-C expression and lymphatic metastasis of breast invasive ductal carcinoma （IDC） and to research the relevance between AKT and VEGF-C,p-AKT and VEGF-C,AKT2 and VEGF-C in IDC. Methods RT-PCR was used to detect the expressions of AKT2 and VEGF-C mRNA on 17 fresh breast cancer tissues.Immunohistochemical （IHC） method （SP） was used to examine the expressions of AKT,p-AKT,AKT2 and VEGF-C protein in paraffin-embedded specimens of 74 breast invasive ductal carcinomas.Western-blot was used to detect the expressions of AKT,p-AKT,AKT2 and VEGF-C protein on 17 fresh breast cancer tissues.Results The expression of AKT2 mRNA in IDC with lymphatic metastasis was higher than that in IDC without lymphatic metastasis（P〈0.05）.And there was no significant difference on the expression of VEGF-C mRNA（P〉0.05）.The difference between the expressions of AKT2 and VEGF-C protein in the two groups were statistically significant （P〈0.05）.There was no significant differences between the expression of AKT and p-AKT protein in the two groups （P〉0.05 ）.The expressions of p-AKT,AKT2 and VEGF-C protein in metastasis group were higher than those in non-metastasis group.There was no significant difference on the expression of AKT protein between two groups.There were significant positive correlations between expression of AKT and VEGF-C,p-AKT and VEGF-C in breast cancer （r=0.283,P=0.015; r= 0.328 ,P=0.004）.There was no significant correlations between expression of AKT2 and VEGF-C in IDC （r=0.072,P= 0.543 ）.Conclusion The expressions of AKT2 and VEGF-C were closely related to lymphatic metastasis of IDC.AKT2 and VEGF-C can be used as predictors of lymphatic metastasis.

关 键 词：乳腺肿瘤 AKT VEGF-C 肿瘤转移 

分 类 号：R737.9[医药卫生—肿瘤] R73-37[医药卫生—临床医学]