喉鳞状细胞癌组织中MUC18的表达及其与微血管和淋巴管计数的关系  

Expression of MUC18 and Its Correlation with Counts of Microvessel and Lymph Vessel in Laryngeal Squamous Cell Carcinoma Tissues

作　　者：李友忠[1] 杨新明[1] 唐青来[1] 

机构地区：[1]中南大学湘雅二院耳鼻咽喉头颈外科,长沙410011

出　　处：《肿瘤防治研究》2010年第7期795-798,共4页Cancer Research on Prevention and Treatment

摘　　要：目的研究喉鳞状细胞癌组织中MUC18表达水平及微血管、淋巴管计数,探讨它们的临床病理意义及在喉鳞状细胞癌中的相互关系。方法 EnVision免疫组织化学法检测62例喉鳞状细胞癌和38例癌旁组织中MUC18的表达,SP免疫组织化学法检测微血管和淋巴管的计数。结果 62例喉鳞状细胞癌MUC18表达阳性率及微血管、淋巴管计数明显高于癌旁组织、差异均有统计学意义(P<0.01)。喉高分化鳞状细胞癌、肿块最大径<2cm、无淋巴结转移及未侵犯周围组织器官病例MUC18表达阳性率及微血管、淋巴管计数明显低于喉低分化鳞状细胞癌、肿块最大径≥2cm、淋巴结转移及侵犯周围组织器官病例,差异有统计学意义(P<0.01);喉声门上型鳞状细胞癌MUC18阳性病例微血管和淋巴管计数明显高于声门型,差异均有统计学意义(P<0.01)。结论 MUC18及微血管、淋巴管计数均可为反映喉鳞状细胞癌进展、转移、侵袭能力及预后的重要生物学标志物,MUC18能促进喉鳞状细胞癌癌组织中血管和淋巴管生成。Objective To study the Expression Levels of MUC18 and the counts of MV and LV in the laryngeal squamous cell carcinoma tissues to detect their relation to the genesis and progression of laryngeal carcinoma.Methods EnVision immunohistochemistry was usd for expression of MUC18 and SP immunohistochemistry for counts of MV and LV in routinely paraffin-embedded sections from the resected specimens with laryngeal squamous cell carcinoma （n=62）,peri-carcinoma tissues （n=38）,chronic laryngitis,and laryngeal papilloma （n=20）.Results The positive rates of MUC18 expression and the counts of MV and LV in laryngeal squamous cell carcinoma were significantly higher than those in peri-carcinoma tissues and chronic laryngitis,laryngeal papilloma （P〈0.01）.The positive rate of MUC18 expression and the counts of MV and LV in well-differentiated laryngeal squamous cell carcinoma with the maximal diameter of mass〈2cm,no-metastasis of lymphnode,and no-infiltration of regional tissues were significantly lower than those in the poor-differentiated laryngeal squamous cell carcinoma with the maximal diameter ≥2cm,metastasis of lymphnode,and infiltration of regional tissues （P〈0.01）.The counts of MV and LV in the supraglottic carcinoma cases were significantly higher were significantly higher than that in glottic carcinoma cases （P〈0.01）.Conclusion The expressive levels of MUC18 and the counts of MV and LV might be important biological marker to determine the progression,metastatic status,invasive potential and prognosis of laryngeal squamous cell carcinoma.MUC18 might have a promotive effect on angiogenesis and lymphangiogenesis in laryngeal squamous cell carcinoma.

关 键 词：喉鳞状细胞癌 血管生成 淋巴管生成 免疫组织化学 

分 类 号：R739.65[医药卫生—肿瘤]