SDF-1/CXCR4轴在白血病患者骨髓中的表达及与血管新生的关系  

Relationship between Expression of SDF-1/CXCR4 Axis and Angiogenesis in Bone Marrow of Patients with Leukemia

作　　者：包萃华[1] 贺其图[2] 李志芹[2] 卢燕[2] 韩轩茂[2] 徐宁[2] 赵志英[2] 刘学文[2] 李静[2] 李喆[2] 马宏杰[2] 云雁[2] 张冬霞[2] 

机构地区：[1]包钢医院血液科,内蒙古包头014010 [2]包头医学院第一附属医院血液科

出　　处：《肿瘤防治研究》2010年第7期822-826,共5页Cancer Research on Prevention and Treatment

基　　金：内蒙古自然科学基金资助项目(200711020921)

摘　　要：目的探讨SDF-1/CXCR4轴在白血病患者骨髓中的表达及与血管新生的关系。方法采用CD45/SSC设门四色流式细胞仪检测55例白血病患者骨髓的CXCR4表达。采用ELISA法检测SDF-1/VEGF;采用Envinson二步法检测骨髓组织MVD;多重PCR法检测白血病患者IgH、TCRγ链V区和J区,采用G-显带技术进行细胞遗传学核型分析。结果 AML组SDF-1(2145.21±329.45)pg/ml、CXCR4阳性表达率(60.01±18.5)%;ALL组SDF-1(2549.02±303.4)pg/ml、CXCR4阳性表达率(70.22±12.73)%;CML组SDF-1(1929.72±253.81)pg/ml、CXCR4阳性表达率(40.05±16.69)%;CRAL组SDF-1(2070.98±159.98)pg/mL、CXCR4阳性表达率(58.4±11.8)%;与对照组相比均明显增高,P<0.05。急淋SDF-1/CXCR4表达率高于其他白血病组;白血病SDF-1/CXCR4表达与相关因素的分析中发现,ALL与外周血WBC数量有相关性(r=0.534、0.567,P<0.01),与急性白血病伴髓外浸润者有意义(P<0.05);SDF-1/CXCR4表达与白血病患者血小板计数、年龄、性别差异、骨髓增生程度、染色体改变、急性白血病骨髓细胞CD34+表达、ALL的IgH和TCRγ链V区和J区基因重排等因素无明显关系。白血病患者骨髓SDF-1、CXCR4、VEGF的相关系数为0.552,0.553,0.531,P<0.05。三者具有显著相关性。结论白血病骨髓液中SDF-1含量及各群细胞CXCR4高表达,其中ALL表达最高;急性白血病缓解后依然高表达;SDF-1/CXCR4表达与ALL的外周血白细胞数量、有髓外浸润者有关,白血病中SDF-1/CXCR4、VEGF高表达并且相互之间存在有相关性,与白血病血管新生有关联。Objective To study the relationship of the SDF-1 level and the expression of CXCR4 axis with the angiogenesis in the bone marrow of patients with leukemia.Methods Four-color multiparametric flow cytometry （FCM） with CD45/SSC gating was used to determine the expression of CXCR4 in the marrow of 55 patients with newly diagnosed leukemia who were chosen as subjects：dipl-antibody ELISA was used to detect SDF-1/VEGF,and the microvessel density （MVD） of bone marrow was determined by immunohistochemical （Envinson） two-step method.Multiple PCR method was used to detect the gene re-arrangement of IgH,TCRγ stains V and J domains; G-differential staining technique was used to carry out cytogentic karyotype analysis.Results In the AML group,SDF-1 is（2145.21±329.45）pg/ml and the positive rate of CXCR4 expression is （60.01±18.5）%;in the ALL group SDF-1 is （2549.1±303.4） pg/ml and positive rate of CXCR4 expression is （70.22±12.73）%; in the CML group SDF-1 is （1929.72±253.8） pg/ml and the positive rate of CXCR4 expression is （40.05±16.69）%; in the CRAL group SDF-1 is （2070.98±159.98） pg/ml and the positive rate of CXCR4 expression is （58.4±11.8）%.The remarkable increase in SDF-1 level and the expression rate of CXCR4 is suggestive of statistical significance （P〈0.05）.In the ALL group SDF-1 level and the expression rate of CXCR4 are higher than those in other groups.In the analysis of the correlated factors and the SDF-1 / CXCR4 expression in leukemia it is found that ALL has a correlation with WBC count in the peripheral blood （r=0.534; 0.567.P〈0.01）.Acute leukemia accompanied by extra-marrow infiltration has statistical significance （P〈0.05）.SDF-1 /CXCR4 expression in leukemia has no correlation with factors such as blood platelets count,age,gender bone marrow proliferation degree,chromosomal change,CD34 positive expression in the marrow cells in acute leukemia,gene re-arrangement in IgH,TCRγ stains V and J domains.The coefficient of correlation between S

关 键 词：白血病 SDF-1/CXCR4轴 血管新生 VEGF 

分 类 号：R733.7[医药卫生—肿瘤]