胆管癌组织中癌细胞增殖、死亡及相关基因检测的临床意义  

Clinical significance of detecting proliferation,apoptosis and necrosis in cholangiocarcinoma tissues

作　　者：黄忠贤 黄宝玉[2] 刘培根[2] 马利林[2] 

机构地区：[1]南通市通州区第三人民医院普通外科,226311 [2]南通大学附属医院普通外科

出　　处：《江苏医药》2010年第13期1531-1533,共3页Jiangsu Medical Journal

摘　　要：目的探讨胆管癌组织中癌细胞增殖、凋亡及坏死特点以及相关基因p16、nm-23、p53和Bcl-2的表达及其临床意义。方法对32例胆总管癌患者术后标本的组织切片作检测,以TdT法测定细胞凋亡指标,HE染色观察组织坏死灶;以LSAB法测定细胞增殖指标和上述四个基因的表达。所有病例作随访。结果四种基因在胆管癌组织中多有不同程度的表达。细胞凋亡、坏死、增殖指数以及死亡/增殖比分别是(0.18±0.45),(0.24±0.67),(0.65±0.36)和(0.69±0.44)。细胞增殖指数低于平均值、细胞凋亡、坏死指数以及死亡/增殖比高于平均值的病例,术后生存时间长。结论胆管癌组织中细胞生长特征是了解患者预后的颇有价值的生物学标志。Objective To investigate the detection and clinical value of proliferation,apoptosis and necrosis in cholangiocarcinoma tissues.Methods The expressions of p16,nm-23,p53 and Bcl-2 were detected and cell proliferation and apoptosis were examined using TdT assay and immunohistochemical method in 32 specimens of adenocarcinoma in the common bile duct.Relationship was analysed between indexes of cell growth or cell loss and survial rate.Results p16,nm-23,p53 and Bcl-2 were all expressed in cancer tissue to a certain extent.The indexes of apoptosis,necrosis,proliferation and the ratio of cell loss to cell growth were （0.18±0.45）,（0.24±0.67）,（0.65±0.36） and （0.69±0.44）,respectively.The patients with higher index of apoptosis and higher ratio of cell loss to cell growth or patients with lower index of cell proliferation had longer survival period.Conclusion The level of cell loss and cell proliferation in cholangiocarcinoma is a valuable marker for predicting prognosis.

关 键 词：胆管肿瘤 增殖 细胞凋亡 

分 类 号：R735[医药卫生—肿瘤]