小鼠视网膜紫外线光损伤中MDA与SOD的作用  

Effect of MDA and SOD on retinal ultraviolet light damage in mice

作　　者：张文华[1] 黄雪桃[1] 唐仁泓[1] 

机构地区：[1]中南大学湘雅三医院眼科,中国湖南省长沙市410013

出　　处：《国际眼科杂志》2010年第8期1490-1492,共3页International Eye Science

摘　　要：目的:探讨视网膜紫外线光损伤中MDA与SOD的作用。方法:取健康成年昆明小鼠30只,随机分成正常对照组和实验组,实验组再分为光损伤后1,2,3,4wk组,每组6只,各组小鼠均在12h明(30～50Lux)12h暗环境中饲养7d,然后暗适应36h。正常对照组不予紫外线照射,实验组小鼠采用2200±138Lux波长为300～400nm的紫外线灯照射,每日连续光照8h。在模型制作完成后摘取眼球,左眼行视网膜匀浆中SOD活力与MDA含量测定。结果:紫外线光照组光照后SOD活力、MDA含量与正常对照组比较差别有统计学意义(P<0.05)。结论:慢性紫外线光损伤与脂质过氧化及自由基的产生有关。·AIM：To approach the effect of the MDA,SOD in the occurrence and development of retinal ultraviolet light damage.·METHODS：Totally 30 Kunming mice were randomly divided into the normal control group and experimental group.The experimental group were divided into the ultraviolet ray illumination 1,2,3,4 weeks groups respec-tively.All mice were raised in cyclic light envioronment for 7 days and then kept in darkness for 36 hours before experiment.The experimental group were exposed to 2200±138Lux illuminations of ultraviolet rays for continu-ously 8 hours every day to establish the model of retinal ultraviolet light damage.The eyes were enucleated after exposured 1,2,3,4 weeks separately the left eyes were examined the SOD activity and the MDA content change of the retinal refining.·RESULTS：There was significant difference of the SOD activity and the MDA content between the control group and the experimental group（P〈0.05）.·CONCLUSION：Lipin peroxidation and free radical pro-duction have a relationship with the retinal ultra-violet light damage.·

关 键 词：视网膜光损伤 紫外线 MDA SOD 

分 类 号：R774.1[医药卫生—眼科]