卡维地洛对动脉粥样硬化大鼠主动脉谷胱甘肽-S-转移酶Mu1表达的影响  

Effect of Carvedilol on Expression of GSTM1 in Atherosclerosis Rats

作　　者：周四喜[1] 袁茜[2] 黄英[1] 宋铁[1] 覃红艳[1] 庄景凡[1] 彭湃[1] 

机构地区：[1]常德职业技术学院医学系机能学教研室,湖南常德415000 [2]中山大学中山医学院临床医学系

出　　处：《实用预防医学》2010年第8期1507-1509,共3页Practical Preventive Medicine

摘　　要：目的观察卡维地洛对动脉粥样硬化大鼠主动脉谷胱甘肽-S-转移酶Mu1(glutathione S-transferase Mu1,GSTM1)表达的影响,探讨卡维地洛抗动脉粥样硬化的作用机制。方法实验分为正常对照组、动脉粥样硬化模型组、卡维地洛治疗组(卡维地洛10mg/kg.d,连续灌胃6周)。实验结束后,观察大鼠血管形态学变化,检测血清超氧化物歧化酶(SOD)、丙二醛(MDA)含量,观察大鼠主动脉GSTM1表达及活性的变化。结果与正常组比较,动脉粥样硬化模型组血清SOD活性降低,MDA含量增加;与动脉粥样硬化模型组比较,卡维地洛治疗组血清SOD活性明显升高,血清MDA含量明显下降。GSTM1的表达及活性在动脉粥样硬化模型组显著降低,而卡维地洛显著增加了GSTM1的表达及活性。结论卡维地洛具有明显的抗动脉粥样硬化作用,这可能与其增加GSTM1表达,从而增强抗氧化作用,消除氧化应激有关。Objective To observe the effect of carvedilol on the expression of glutathione S-transferase Mu 1（GSTM1）in atherosclerosis rats,and to explore the mechanism of carvedilol action in atherosclerosis.Methods There were 3 groups in the experiment：normal control group,atherosclerosis model group,carvedilol treatment group（10 mg/kg.d,for 6 weeks）.After the end of the experiment,the change of vascular morphology was observed,and the superoxide dismutase（SOD）activity and malondialdehyde（MDA）level were determined.The changes of expression and activity of GSTM1 were investigated by Western blot.Results As compared with the age-matched control rats,the SOD activity was decreased and the level of MDA was increased in atherosclerosis rats.In comparison with atherosclerosis rats,the SOD activity was significantly increased and the level of MDA was significantly decreased in rats with carvedilol treatment.The expression and activity of GSTM1 were significantly decreased in atherosclerosis rats,but they were significantly increased by carvedilol.Conclusions Carvedilol has the effect of anti-atherosclerosis,which might be associated with the up-regulation of GSTM1 expression,the enhancement of antioxidation role,and the elimination of oxidative stress.

关 键 词：卡维地洛 动脉粥样硬化 谷胱甘肽-S-转移酶Mu1 

分 类 号：R-332[医药卫生]