无环鸟苷对K562细胞的抗增殖和诱导分化作用  

The effect of acycloguanosine on inhibiting proliferation and inducing differentiation of K562 cells

作　　者：居小萍[1] 袁有忠[1] 韩军[1] 夏放[1] 孟沛霖[1] 

机构地区：[1]第二军医大学长海医院

出　　处：《第二军医大学学报》1999年第1期18-19,共2页Academic Journal of Second Military Medical University

摘　　要：目的：探讨无环鸟苷（ＡＣＶ）对人白血病细胞的抗增殖和诱导分化作用。方法：将无环鸟苷加入Ｋ５６２细胞培养４ｄ，测定其活细胞数目、联苯胺染色阳性率，光镜观察形态、组织化学染色。结果：在ＡＣＶ的作用下，Ｋ５６２细胞的增殖受抑，生长分数降低，并且向具有合成血红蛋白能力的细胞分化。结论：ＡＣＶ对Ｋ５６２细胞具有抑制增殖和诱导分化作用，该结果可为慢性粒细胞白血病的治疗探索新的途径。Objective: To explore whether acycloguanosine (ACV) can inhibit the proliferation and induce the erythrodifferentiation of human myeloid leukemia K562 cells. Methods: K562 cells were cultured with ACV for 4 d to detect cellular changes. Results: When K562 cells grew in a medium containing ACV, the cellular growth and division were gradually suppressed, growth fraction decreased and further differentiation towards the cell producing hemoglobins was found. Conclusion: ACV can inhibit proliferation and induce erythrodifferentiation of K562 cells.

关 键 词：无环鸟苷 慢粒性 白血病 K562细胞 诱导分化 

分 类 号：R733.720.5[医药卫生—肿瘤] R730.53[医药卫生—临床医学]