胃癌细胞株蛙皮素受体的检测  

The expression of bombesin receptor in gastric cancer

作　　者：孙涛[1,2] 陈源稼[1,2] 侯敏[1,2] 李晓波[1,2] 陈元方[1,2] 

机构地区：[1]中国协和医科大学,北京协和医院消化内科 [2]海军总医院消化内科

出　　处：《中华内科杂志》1999年第2期85-87,共3页Chinese Journal of Internal Medicine

摘　　要：目的观察胃癌细胞株ＳＧＣ７９０１细胞蛙皮素受体ｍＲＮＡ和受体蛋白的表达。方法（１）利用逆转录聚合酶链反应（ＲＴＰＣＲ）和Ｓｏｕｔｈｅｒｎｂｌｏｔ观察胃癌细胞株ＳＧＣ７９０１细胞蛙皮素受体ｍＲＮＡ的表达；（２）利用交联剂（ＤＳＳ）化学交联增强化学发光（ＥＣＬ）自显影观察胃癌细胞株ＳＧＣ７９０１细胞株受体蛋白的表达。结果（１）ＲＴＰＣＲ产物经Ｓｏｕｔｈｅｒｎ杂交与预期的蛙皮素受体ｃＤＮＡ分子量完全相符。（２）ＤＳＳ化学交联ＥＣＬ自显影证明胃癌细胞ＳＧＣ７９０１细胞存在特异性蛙皮素受体蛋白，其分子量约７５０００，且具有很好的结合解离曲线。结论我们的实验证明了此株细胞存在有蛙皮素／胃泌素释放肽特异性受体。本研究为探索蛙皮素和蛙皮素受体的拮抗剂应用于肿瘤研究以及蛙皮素作用后细胞内信息传递途径提供了基础。Objective To observe the expression of receptor mRNA and its protein in SGC 7901 cells. Methods (1)RT PCR and Southern blot hybridization were performed for detection of gastrin releasing peptide GRP R mRNA; (2) Covalent cross linking study with biotinyl bombesin and enhanced chemoluminescence(ECL) was performed to identify GRP R at protein levels in SGC 7901 cells. Results (1) It is shown that the molecular weight of the cDNA was consistent with that of expected. Southern blot was then used to further prove that specific GRP R mRNA was expressed by SGC 7901. (2) GRP R protein was shown by covalent cross linking in SGC 7901 and the molecular weight of bombesin/GRP R in SGC 7901 cells was about 75 000. Conclusion By using RT PCR, Southern blot and covalent cross linking techniques, it was demonstrated that SGC 7901 cells can express bombesin receptor mRNA and protein. The results of this study provides a basis for further researches on bombesin intracellar signal transmission pathway and the role of bombesin and bombesin receptor antagnonists in oncological research

关 键 词：聚合酶链反应 胃肿瘤 细胞株 蛙皮素 受体 

分 类 号：R735.202[医药卫生—肿瘤]