1,3-丁二烯作业工人XRCC4基因多态性与染色体损伤的关系  

Association of XRCC4 polymorphisms and chromosomal damage levels in 1,3-butadiene workers

作　　者：刘楠[1] 关维俊[1] 孟会林[2] 崔涛[2] 李忠生[2] 程娟[2] 肖经纬[2] 王学生[1] 李斌[2] 

机构地区：[1]华北煤炭医学院预防医学系国家河北省煤矿卫生与安全实验室,唐山063000 [2]中国疾病预防控制中心职业卫生与中毒控制所

出　　处：《卫生研究》2010年第4期407-411,共5页Journal of Hygiene Research

基　　金：国家自然科学基金资助项目(No.30471471)

摘　　要：目的探讨DNA双链断裂修复酶XRCC4基因多态性与1,3-丁二烯(BD)作业工人染色体损伤易感性的关系。方法选取189名BD作业工人和83名行政人员作为研究对象,采用(CBMN)评价个体外周血淋巴细胞染色体损伤水平,用聚合酶链反应-限制性片段长度多态性方法分析XRCC4基因3个单体型标签单核苷酸多态性位点(A245G、T1394G和C1475T),Clark运算法确定个体XRCC4基因的单体型等位基因和单体型对,协方差分析校正相关影响因素,比较具有不同XRCC4基因型或单体型对的个体外周血淋巴细胞CBMN率的差异。结果在校正年龄、性别、吸烟、饮酒影响因素后,BD作业工人CBMN率为(7.17±5.41)‰、核质桥(NPB)率为(1.20±1.43)‰、核芽(NBUD)率为(2.87±2.44)‰,对照组的CBMN率为(3.10±2.56)‰、NPB率为(0.24±0.66)‰、NBUD率为(1.68±1.92)‰,差异有显著性(P<0.01);暴露组中XRCC4A245G位点AA基因型个体NPB率显著高于AG基因型或GG基因型个体(P<0.05);按年龄分层<39岁组中XRCC4T1394G位点TT基因型个体CBMN率高于GG基因型个体(P<0.01)。结论 XRCC4A245G和T1394G位点的多态性能影响职业性BD暴露导致的外周血淋巴细胞染色体损伤的易感性。Objective To investigate the association of polymorphisms of DNA double-strand break repair gene XRCC4 and chromosomal damage levels in peripheral blood lymphocyte in workers exposed to 1,3-butadien（ BD）.Methods 189 BD exposed workers and 83 controls were recruited in this study.Chromosomal damage in peripheral lymphocytes was measured by the cytokinesis-block micronucleus （ CBMN ） assay.Three haplotpe-tagging single nucleotide polymorphisms in XRCC4 gene,including A245G,T1394G and C1475T,were detected by PCR-RFLP and the XRCC4 haplotypes were estimated by using an extension of Clark algorithm.The associations between haplotype pairs and micronuclei data were assessed by analysis of covariance in the exposed and non-exposed groups.Results Multivariate analysis of covariance revealed that the frequencies of CBMN,nucleoplasmic bridge （NPB） and nuclear bud （NBUD） were significantly higher in BD exposed workers [（7.17 ± 5.41） ‰,（1.20 ± 1.43） ‰ and （2.87 ± 2.44） ‰,respectively]than in the controls [（3.10 ± 2.56） ‰,（0.24 ± 0.66） ‰ and （1.68 ± 1.92） ‰,P〈0.01,respectively].Rank sum test revealed that in BD exposed workers,the XRCC4 A245G AA genotype exhibited higher NPB frequency than that of AG or GG genotypes significantly（ P〈0.05）.Stratification analysis showed that the polymorphism of XRCC4 T1394G TT carriers had significantly higher CBMN frequencies than those GG carriers （ P〈0.01 ） in 〈39 year workers.Conclusion The results suggested that polymorphisms of XRCC4 A245G and T1394G could influence the chromosomal damage levels in BD exposed workers.

关 键 词：1 3-丁二烯 XRCC4基因 基因多态性 单体型 胞质分裂阻滞微核试验 染色体损伤 

分 类 号：Q343.12[生物学—遗传学]