叶黄素对D-半乳糖致氧化损伤小鼠HO-1、TLR4表达的影响  

Effect of lutein on relieving oxidative stress in mice induced by D-galatose

作　　者：麦嘉仪[1] 沈新南[1] 施冬云[2] 魏延[1] 沈慧[1] 吴岷[1] 

机构地区：[1]复旦大学公共卫生学院营养与食品卫生学教研室公共卫生安全教育部重点实验室,上海200032 [2]复旦大学上海医学院生物化学与分子生物学系

出　　处：《卫生研究》2010年第4期430-432,共3页Journal of Hygiene Research

基　　金：上海市科学技术委员会科研计划项目(No.08PJ14018)

摘　　要：目的研究叶黄素缓解D-半乳糖诱导小鼠氧化应激损伤的作用机制。方法 48只昆明种小鼠随机分为D-半乳糖模型对照组、叶黄素低剂量组(10mg/kg·d)、叶黄素高剂量组(40mg/kg·d)和正常对照组。6周后观察小鼠肝组织活性氧(ROS)、一氧化氮(NO)含量,总一氧化氮合酶(TNOS)、诱导型一氧化氮合酶(iNOS)和线粒体ATP酶(ATPase)活性,RT-PCR法检测血红素氧合酶(HO-1)和toll样受体4(TLR4)mRNA表达水平。结果叶黄素低、高剂量组小鼠肝组织ROS含量低于模型对照组(P<0.01);低、高剂量组线粒体Na+-K+-ATP酶及高剂量组线粒体Ca2+-ATP酶活性高于模型对照组(P<0.05);高剂量组TNOS、iNOS活性及NO含量低于模型对照组(P<0.05);高剂量组小鼠肝HO-1mRNA表达高于模型对照组(P<0.05),叶黄素低、高剂量组TLR4mRNA表达均低于模型对照组(P<0.01)。结论叶黄素降低D-半乳糖小鼠氧化应激效应可能与其提高HO-1表达和下调TLR4mRNA表达有关。Objective To study the effect of lutein on relieving oxidative stress in the liver of mice induced by D-galactose（D-gal）.Methods Forty eight Kunming strain mice were randomized into 4 groups： model group,low lutein group （LL 10 mg /（kg·d））,high lutein group（HL 40 mg /（kg·d）） and normal control group.The content of reactive oxygen species （ROS）,nitric oxide （NO） and activity of total nitric oxide synthase （TNOS）,inducible nitric oxide synthase （ iNOS） and mitochondrial Na ^＋ -K^ ＋ -ATPase,Ca^2 ＋ -ATPase in liver tissue were detected 6 weeks later in the experiment.The expression of toll-like receptor-4 （TLR4） mRNA and hemeoxygenase-1 （HO-1） mRNA in hepatic tissue were detected by reverse transcription polymerase chain reaction （ RT-PCR） technique.Results ROS content in HL and LL group was significantly lower（P〈0.01） than that in the model group.The activity of Na ^＋ - K^ ＋ -ATPase in HL and LL group and the activity of Ca^2 ＋ -ATPase in HL group were significantly higher than that in the model group （ P〈0.05）.The activities of TNOS and iNOS and the content of NO in HL group were significantly lower than the model group （P〈0.05）.The expression of HO-1 mRNA in HL group was significantly higher than that in the model group,but the expression of TLR4 mRNA in HL group was significantly lower than that in the model group （ P〈0.05）.Conclusion The mechanism of lutein on the protection of mice from oxidative stress induced by D-gal might be related to increasing the expression of HO-1 mRNA and reducing the expression of TLR4 mRNA.

关 键 词：叶黄素 氧化应激 诱导型一氧化氮合酶 血红素氧合酶 toll样受体4 D-半乳糖 

分 类 号：Q562[生物学—生物化学] Q523.3