TNFα对LAK细胞抗胃癌效应的影响  

Influences of TNFα on effectiveness of LAK cells on gastric cancer

作　　者：刘苏[1,2] 张兴荣[1,2] 章永平 吴裕忻[1,2] 

机构地区：[1]第二军医大学长征医院消化内科 [2]上海第二医科大学瑞金医院消化研究室

出　　处：《第二军医大学学报》1999年第2期102-104,共3页Academic Journal of Second Military Medical University

摘　　要：目的：观察体外ＴＮＦα联用ＩＬ－２培养ＬＡＫ细胞的抗胃癌作用及ＴＮＦα预处理后胃癌细胞对ＬＡＫ细胞敏感性的变化。方法：以不同浓度ＴＮＦα与ＩＬ－２配伍培养ＬＡＫ细胞，并做ＴＮＦα的单抗阻断试验，用ＬＤＨ释放法检测ＬＡＫ细胞抗癌活性；以ＴＮＦα预处理胃癌细胞，检测ＬＡＫ细胞抗瘤活性。结果：ＴＮＦα在低浓度ＩＬ－２下增加ＬＡＫ活性，使ＩＬ－２诱导同等ＬＡＫ活性的浓度下降约１０倍；ＴＮＦα预处理使ＬＡＫ对其抗瘤活性提高约９％。结论：ＴＮＦα可增加ＩＬ－２诱导的ＬＡＫ抗胃癌活性；Objective: To investigate the anti gastric cancer (GC) effect of LAK cells ind uced by TNFα with IL 2 and the influences of pretreatment with TNFα on the susceptibility of GC cells to LAK. Methods: Induced by TNFα combi ned with IL 2 at different concentrations, blocked by TNFα McAb, and after p retreating the GC cells with TNFα, LAK cytotoxicity to GC cells was detected by LDH colometric assay. Results: At low concentrations of IL 2, TNFα increased the cytotoxicity of LAK and the concentration of IL 2 neces sary to induce the cytotoxicity to the same extent decreased 10 folds. Pretrea tment of GC cells with TNFα made the cytotoxicity of LAK to the GC increase by 9 %. Conclusion: TNFα increased the cytotoxicity of LAK induced by IL 2 to GC cells, and the GC cells pretreated with TNFα were more susceptible t o LAK.

关 键 词：肿瘤坏死因子 胃肿瘤 LAK细胞 

分 类 号：R735.2[医药卫生—肿瘤]