两种食管鳞癌细胞系增殖和侵袭能力的比较研究  

Comparative study on the proliferation and invasion of two esophageal squamous carcinoma cell lines

作　　者：谢仰民[1] 董辉[2] 谢剑君[2] 吴智勇[2] 杜则澎[3] 李利艳[3] 许秀娥[3] 周飞[1] 陈涛[2] 石宏顺[3] 许丽艳[3] 李恩民[2] 

机构地区：[1]汕头大学医学院实验动物中心 [2]汕头大学医学院生物化学与分子生物学教研室 [3]汕头大学医学院肿瘤病理研究室,广东汕头515041

出　　处：《癌变．畸变．突变》2010年第4期261-264,共4页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：广东省科技计划项目(2009B060300016);NSFC-广东省联合基金重点项目(U0932001)

摘　　要：目的:研究两种来源不同的食管鳞癌细胞系EC109和KYSE510分裂增殖能力和侵袭能力的差异。方法:利用MTT实验和侵袭实验,在体外分别检测EC109和KYSE510细胞的分裂增殖能力和侵袭能力;采用裸鼠皮下接种实验和爪垫皮下接种淋巴结转移模型,比较两种细胞在体内的成瘤能力、局部侵袭能力和区域淋巴结转移能力的差异;免疫印迹检测上皮-间充质细胞转换标志蛋白E-cadherin、γ-cadherin和Vimentin在两种细胞中的表达水平。结果:体外实验表明,EC109细胞的分裂增殖和侵袭能力均明显较KYSE510细胞的强(P<0.05);皮下接种和爪垫皮下接种淋巴结转移实验显示EC109细胞的成瘤能力、局部侵袭能力和淋巴结转移能力均较KYSE510细胞的高;免疫印迹检测发现,E-cadherin和γ-cadherin在EC109细胞中的表达水平较KYSE510细胞中的表达低,而Vimentin在EC109细胞中的表达则较KYSE510细胞中的表达高,提示EC109细胞发生上皮-间充质细胞转换的程度较KYSE510的高。结论:EC109细胞的增殖和侵袭能力均较KYSE510细胞的强,上皮-间充质细胞转换可能是导致这种差异的原因之一。OBJECTIVE：This study investigated the proliferation and invasiveness of two different esophageal squamous carcinoma（ESCC）cell lines,EC109 cells and KYSE510 cells.METHODS：MTT assay and invasiveness assay were used to explore the proliferation and invasiveness of EC109 cells and KYSE510 cells in vitro.Then,tumor formation assay in the nude mice was employed to analyze the tumorigenesis and footpad tumor model was used to assess the metastasis in lymph nodes of the two cell lines.Finally,the expressions of E-cadherin,γ-cadherin and Vimentin were detected to estimate the involvement of epithelial-mesenchymal transition（EMT）.RESULTS：In vitro experiments showed that EC109 cells proliferated faster than KYSE510 cells and the invasiveness of EC109 cells was higher than that of KYSE510 cells.The tumorigenesis of EC109 cells was greater than KYSE510 and the metastasis in lymph nodes was found more frequently in EC109 cells.Furthermore,expressions of E-cadherin and γ-cadherin were down-regulated in EC109 cells as compared to KYSE510 cells,while the level of Vimentin was up-regulated.CONCLUSION：EC109 was a ESCC cell line with high potential of proliferation and invasiveness and KYSE510 was not.The difference in proliferation and invasiveness between these two cell lines might be mediated by EMT.

关 键 词：食管癌细胞 细胞增殖 细胞侵袭 转移 

分 类 号：Q71[生物学—分子生物学] R735.1[医药卫生—肿瘤]