草苔虫内酯对SD大鼠致畸作用的研究  

Study on teratogenicity of bryostatin in SD rats

作　　者：马玺里[1] 朱玉平[1] 郑怡[1] 朱江波[1] 毕洁[1] 张天宝[1] 

机构地区：[1]第二军医大学卫生毒理学教研室,上海200433

出　　处：《癌变．畸变．突变》2010年第4期317-319,322,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨草苔虫内酯对SD大鼠的致畸作用。方法:采用大鼠标准致畸试验,孕SD大鼠随机分为4组,每组16～17只。草苔虫内脂高、中、低剂量实验组分别按0.016、0.008、0.004mg/kg给药,对照组给予0.9%生理盐水。大鼠于孕期第6～15天按10ml/kg体重连续尾静脉注射给药。孕20天处死孕鼠,检查母体妊娠与胎鼠畸形情况。结果:中、高剂量组的孕鼠增重分别为67.72、11.98g,与对照组比较明显减少(P<0.05),并具有一定的剂量_反应趋势。在高剂量下,活胎率为61.42%,与对照组比较明显降低(P<0.05);吸收胎率和死胎率分别为20.81%、17.77%,与对照组比较明显增高(P<0.05)。各受试剂量下,活胎体质量、活胎身长较对照组明显减少和降低(P均<0.05)。各实验组均未观察到胎鼠明显的外观、脏器以及骨骼的畸形。结论:草苔虫内酯在中、高剂量下具有母体毒性,在高剂量下有胚胎毒性。在受试剂量下有一定的胎儿毒性,但无明显的致畸作用。OBJECTIVE：To study the teratogenicity of bryostatin in SD rats.METHODS：A standard teratogenicity test was performed to investigate the teratogenicity of bryostatin.Pregnant SD rats were divided into 4 groups（n=16 or 17 for each group）,three bryostatin dosage groups（0.016,0.008,0.004 mg/kg）and one negative control group.Bryostatin or normal saline was given via caudal vein injection from 6th to 15th day of gestation.Pregnant rats were killed at 20th day of gestation,and the parents and their fetuses were examined.RESULTS：The weight gain of pregnant rats during gestation in middle and high dose groups was 67.72 and 11.98g,showing obvious decrease compared with control group.It also revealed a certain dose-response relationship.At the high dose,live fetus rate was 61.42%,which was significantly decreased compared with control group.Absorbed fetus rate and dead fetus rate were 20.81% and 17.77%,respectively,demonstrating obvious increases.There were significant differences between three treatment groups and control group in fetal body length and weight.Bryostatin didn＇t induce any teratogenic effect on the appearance,viscera and skeleton of the fetuses in three bryostatin dosage groups.CONCLUSION：Bryostatin at the dose of 0.016 and 0.008 mg/kg induced matenal toxicity;at the dose of 0.016 mg/kg showed embryotoxicity;at the dose of 0.016,0.008,0.004 mg/kg exerted a certain fetotoxicity,but had no apparent teratogenesis in SD rats.

关 键 词：草苔虫内酯 母体毒性 致畸作用 胚胎毒性 胎儿毒性 

分 类 号：R730.45[医药卫生—肿瘤]