SPME-GC-MS法快速检测尿液中的甲基苯丙胺  

Rapid detection of methamphetamine in urine by SPME-GC-MS

作　　者：李学博[1,2] 周海梅[1] 李朴[1] 马锦琦[1] 程永学 吕宙[1] 齐孝蕊[1] 莫耀南[1] 

机构地区：[1]河南科技大学法医学院,河南洛阳471003 [2]沂南县公安局,山东沂南276300 [3]临沂市公安局,山东临沂276000

出　　处：《中国法医学杂志》2010年第4期252-254,共3页Chinese Journal of Forensic Medicine

摘　　要：目的建立SPME-GC-MS快速检测吸毒人员尿液中的甲基苯丙胺的方法。方法以SPME法提取尿液中的甲基苯丙胺,以1-萘胺作内标,用GC-MS法检测。结果在2～2000ng/mL的范围内呈线性关系(r=0.9985,n=7),甲基苯丙胺的检测限为0.5ng/mL(信噪比>3),在低、中、高(200、500、1000ng/mL)浓度的平均相对回收率为102.6%、98.5%、93.2%,日内及日间RSD分别小于8.1%、7.2%。结论用此方法检测尿液中的甲基苯丙胺,灵敏度高,简单快速,易操作,适用于吸毒人员的快速定性定量检测。Objective To establish a rapid determination method of methamphetamine in urine of druggers by SPME-GC-MS.Methods The methamphetamine in urine was extracted by SPME and detected using GC-MS with 1-Naphthylamine as internal standard.Results The standard curve was linear in the range of 2～2000ng/mL（r=0.9985,n=7）,and the detection limit of methamphetamine was 0.5ng/mL（signal to noise ratio3）,and the average relative recoveries were 102.6%,98.5%,93.2% in low,medium,high concentrations（200,500,1000 ng /mL） respectively,The precisions（RSD） of intra-day and intre-day were less than 8.1% and 7.2%.Conclusion The detection method is high sensitivity,simple and rapid,and it is easy-to-use and suitable for qualitative and quantitative in the fast determination of drug addicts.

关 键 词：法医毒物分析 固相微萃取 GC-MS 甲基苯丙胺 

分 类 号：D919.8[医药卫生—法医学]