自身免疫性肝炎患者外周血CD4＋CD28-T细胞群的检测及意义  

Expression and significance of peripheral blood CD4 ＋ CD28 - T cells in patients with autoimmune hepatitis

作　　者：汪群英[1] 徐芸[1] 

机构地区：[1]郑州大学第一附属医院消化科,450052

出　　处：《中华全科医师杂志》2010年第8期560-562,共3页Chinese Journal of General Practitioners

摘　　要：以二色流式细胞术检测自身免疫性肝炎（AIH）患者59例（活动期36例,缓解期23例）及健康者30例外周血CD4＋CD28-T细胞群的比例,以双抗体夹心ELISA法检测IL-10、肿瘤坏死因子α（TNF-α）水平,结合患者血清免疫球蛋白含量等进行分析.结果 示AIH活动期患者外周血CD4＋CD28-T细胞群的比例和TNF-α较健康者显著升高（均P＜0.05）,而缓解期患者CD4＋CD28-T较健康者略有升高;同一患者活动期经过治疗病情缓解后,外周血CD4＋CD28-T细胞降低（P＜0.05）,而IL-10显著升高（P＜0.05）;复发患者外周血CD4＋CD28-T细胞群的比例明显高于初发患者和健康者;血清免疫球蛋白含量异常者外周血CD4＋CD28-T细胞群的比例明显高于免疫球蛋白正常者及健康者.提示AIH患者外周血CD4＋CD28-T细胞群比例的异常和细胞因子异常与疾病的病程和临床表现相关联,可能参与了AIH疾病的致病机制.The percentage of peripheral blood CD4 ＋ CD28 - T cell in 59 patients with autoimmune hepatitis （AIH） （36 with active disease and 23 stable） and 30 normal individuals was evaluated by twocolor flowcytometry. IL-10 and TNF-α levels were assayed by double-antibody sandwich ELISA. Serum immunoglobulin were also analyzed. Patient with active AIH had significantly higher peripheral blood CD4 ＋ CD28 - T cell and TNF-α levels than normal individuals （ P 〈 0. 05 ）, while in patients with remission they were only slightly higher than normal controls. If remission was induced in patients with active AIH,peripheral blood CD4 ＋ CD28 - T cell percentage would be significantly reduced （ P 〈 0. 05 ） while IL-10 increased （ P 〈 0. 05 ）. The percentage of peripheral blood CD4 ＋ CD28 - T cell in patients with relapse was significantly higher than newly diagnosed patients and normal controls; Peripheral blood CD4 ＋ CD28 -T cell percentage in patients with abnormal serum immunoglobulin （ IgA, IgG and IgM ） levels was significantly higher than those with normal levels and normal controls. We concluded that abnormal peripheral blood CD4 ＋ CD28- T cells and cytokine in patients with AIH may be related to disease activity and clinical manifestations, which may also play a role in the pathogenesis of AIH.

关 键 词：肝炎 自身免疫性 T淋巴细胞 

分 类 号：R575.1[医药卫生—消化系统]