肝癌组织生长抑素受体亚型的表达及其意义  

Differential expression of somatostatin receptor subtypes in hepatocellular carcinoma

作　　者：杨少奇[1] 武曼群[2] 李昭宇[1] 杨力[1] 王琦[1] 赵进[2] 魏来[3] 

机构地区：[1]宁夏医科大学附属医院消化内科/宁夏消化疾病研究所,银川市750004 [2]宁夏医科大学附属第二医院,银川市750004 [3]北京大学肝病研究所

出　　处：《实用肝脏病杂志》2010年第4期288-290,共3页Journal of Practical Hepatology

基　　金：宁夏自然科学基金资助项目(NZ0556)

摘　　要：目的研究原发性肝癌患者肝癌组织中生长抑素受体亚型(SSTR2、3和5)的表达情况及其意义。方法应用免疫组化法检测50例原发性肝癌患者癌组织、癌旁组织以及24例非癌肝组织中SSTR2、3和5的表达;采用RT-PCR法检测10例肝癌组织及非癌肝组织SSTR2、3和5mRNA的表达。结果肝癌组织中SSTR2、3和5蛋白表达阳性率分别为72%、34%、14%,SSTR2的表达率明显高于SSTR3和5,差异有统计学意义(P<0.05);SSTR2在肝癌组织中表达率较非癌肝组织高(x2=7.372,P<0.01),差异有统计学意义;肝癌组织SSTR2、3和5mR-NA表达率分别为100%、80%和30%,均高于非癌肝组织。结论肝癌组织SSTR亚型表达增高,SSTR2为肝癌组织中主要表达的生长抑素受体亚型。Objective To investigate the differential expression of somatostatin receptor subtypes（SSTR）and the major subtypes in hepatocellular carcinoma （HCC）. Methods Immunohistochemistry were used to detect protein expressions of SSTR2,3,5 in 50 HCC and 24 normal liver tissue,and SSTR mRNA were detected in 10 HCC tissues by RT-PCR. Results The positive rate of SSTR2,3,5 protein in HCC tissues were 72%,34%,14%, respectively;The expression of SSTR2 in HCC were markedly higher than in non-HCC liver tissues;The positive rate of SSTR2,3,5 mRNA were 100%,80% and 30%,higher than in non-HCC liver tissues. Conclusion The expression of SSTR subtype is higher in HCC than in non-HCC liver tissues. SSTR2 is the main subtypes expressed in HCC tissues.

关 键 词：肝癌 生长抑素受体 免疫组化 RT-PCR 

分 类 号：R735.7[医药卫生—肿瘤]