阿司匹林、盐酸氟桂利嗪联合预处理对高血糖SD大鼠局部脑缺血再灌注损伤的保护作用  

Research for protective effect of pretreatment by combination of aspirin and flunarizine hydrochlorid on focal cerebral ischemia reperfusion damage in SD rat with hyperglycemia

作　　者：孙文芳[1] 高政[1] 孙艳[1] 姜丽杰[1] 

机构地区：[1]辽宁省大连医科大学附属二院北院神经内科,116031

出　　处：《中国临床实用医学》2010年第8期16-17,共2页China Clinical Practical Medicine

摘　　要：目的采用高血糖条件下Sprague-Dawley（SD）大鼠局灶性脑缺血再灌注模型，观察神经功能缺损评分、脑梗死体积及脑组织病理形态改变，探讨阿司匹林联合盐酸氟桂利嗪预处理对高血糖SD大鼠局灶性脑缺血再灌注损伤是否有保护作用。方法36只雄性健康SD大鼠，建立高血糖模型后随机分为2组：对照组（n=18）、阿司匹林＋盐酸氟桂利嗪组（治疗组，n=18），各组按脑缺血90min再灌注3h（n=6）、6h（n=6）、24h（n=6）分为3个亚组。比较两组在相同再灌注各时间点神经功能缺损评分、脑梗死体积和脑组织病理形态的改变。结果治疗组相同再灌注各时间点神经功能缺损评分好于对照组（P〈0．05）；治疗组脑梗死体积明显小于对照组（P〈0．01）；治疗组与对照组相比，变性、坏死的神经元减少，空泡化改变减轻，组织间水肿减轻。结论阿司匹林联合盐酸氟桂利嗪预处理能减轻高血糖条件下的局灶陛脑缺血再灌注损伤，减轻神经功能缺损症状，缩小梗死体积，减轻神经细胞变性、坏死及组织水肿。Objective Applying focal ischemia-reperfusion model of SD rat on the condition of hyperglycemia,through observing the state of neurologic impairment score, cerebral pathomorphology and infarction volume after focal ischemia-reperfusion damage in SD rat on the condition of hyperglycemia. Methods 36 healthy male SD rats（ weight from 180 g to 220 g）with normal blood glucemia（ 〈 6 mmol/1）were randomly divided into 2 groups ： control group（ n = 18 ） and flunarizine ＋ hyperglycemia group（ treatment group n = 18 ）. Each group was divided into 3 subgroups according to reperfusion 3 h （n = 6）, 6 h （n = 6）, 24 h（ n = 6 ）after ischemia for 90 minutes. Compare the differences of neurologic impairment score, cerebral infarction volume and pathomorphology. Results There are great difference of the neurologic impairment score between the two groups ,P 〈 0.05. The infarction volume in the treatment group was lower than that in the control group, P 〈 0. 01. Cerebral tissue pathomorphology：In treatment group, the injury of cerebral tissue became serious with time went by, but compared with control group, the number of neuron which became degeneration and necrosis decreased, vacuolization and intertissue edema became relieved. Conclusion On the condition of hyperglycemia,pretreatment of aspirin and flunarizine hydrochlorid could decrease focal ischemia-reperfusion damage, relieve the neurologic impairment symptoms;reduce infarction volume, reduce the neuron degeneration, necrosis and tissue edma.

关 键 词：脑缺血再灌注 神经功能缺损 阿司匹林 盐酸氟桂利嗪 高血糖 

分 类 号：R743.3[医药卫生—神经病学与精神病学]