不同相对分子质量软骨多糖对K562细胞的增殖抑制作用  

Inhibition of K562 cell proliferation by cartilage polysaccharide with different relative molecular mass

作　　者：杨宁[1] 刘安军[1] 宋微[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院,天津300457

出　　处：《中国科技论文在线》2009年第3期223-226,共4页

基　　金：国家自然科学基金(20776113);高等学校博士学科点专项科研基金(20050057002)

摘　　要：研究不同相对分子质量的软骨多糖对慢性髓系红白血病细胞K562的增殖抑制作用。用不同浓度的酒精对软骨提取物进行分级沉淀,得到不同分子质量的软骨多糖,并用聚丙烯酰胺凝胶电泳(SDS-PAGE)检测所得软骨多糖的纯度及分子质量;采用噻唑蓝(MTT)比色法测定细胞的增殖活性;用琼脂糖凝胶电泳法检测DNA电泳特征。结果表明,短链软骨多糖(相对分子质量约为3×104u)对K562细胞具有明显的增殖抑制作用,这种抑制作用呈现明显的时间依赖性,且琼脂糖凝胶电泳出现明显的DNA梯状条带。In order to study the proliferation of chronic myegenous leukemia（K562） treated by different relative molecular mass cartilage polysaccharide（sc-PS）,cartilage extract was precipitated by ethanol with different concentration.Different relative molecular mass polysaccharides were collected.Then concentration and molecular mass of them were studied by SDS-PAGE;MTT method was used to test the proliferation of cells treated by sc-PS,and agarose gel electrophoresis was used to detect the characteristic of DNA.The results show that the growth of K562 cells is inhibited obviously by sc-PS,which in a dose-dependent,DNA ladder could be observed apparently in agarose gel electrophoresis.

关 键 词：软骨多糖 K562细胞 MTT法 DNA电泳 

分 类 号：R733.7[医药卫生—肿瘤]