Vgb在大肠埃希菌中表达及生物活性的研究  

Study on the expression and activity of vgb in recombinant Escherichia coli

作　　者：邢安辉[1] 王洪军[1] 尹旭辉[1] 王立强[1] 于宁[1] 孙艳[1,2] 张锐[1,3] 

机构地区：[1]沈阳军区联勤部疾病预防控制中心,辽宁沈阳110034 [2]沈阳药科大学,辽宁沈阳110016 [3]辽宁渥尔夫房地产开发有限公司,辽宁沈阳110034

出　　处：《中国微生态学杂志》2010年第7期599-601,605,共4页Chinese Journal of Microecology

基　　金：辽宁省教育厅青年人才基金资助课题(05L433)

摘　　要：目的将vgb克隆到大肠埃希菌表达载体pQE-30中,研究vgb表达产物对细胞摄氧能力的影响。方法利用PCR和基因重组技术克隆vgb与大肠埃希菌表达质粒pQE V,大肠埃希菌转化采用CaCl2法,VHb活性分析采用CO示差光谱法。结果克隆了vgb和重组质粒pQE V,vgb基因在大肠埃希菌中获得表达,在A420 nm处达到典型吸收峰。结论 vgb在大肠埃希菌中表达产物加强了对氧的摄取能力,对解决细胞高密度发酵培养中氧需矛盾、促进代谢产物的产率具有非常重要的应用价值。Objective To study the effect of vgb expression product on cell respiration capability by cloning vgb gene into the expression vctor pQE-30 of Escherichia coli.Method Vgb was cloned by PCR;plasmid pQE V was constructed by gene recombinant technology.Cotransformation of Escherichia coli was conducted by CaCl2 method.The activity of VHb was examined by CO difference spectrum.Result Vgb was cloned and plasmid-pQE V was reconstructed successfully.Vgb gene had been expressed in Escherichia coli and a typical peak of the expression product had been obtained at A420 nm.Conclusion The vgb expression product in Escherichia coli can improve the oxygen intake capacity of the bacteria.This may have an extremely important value for mainteining oxygen supply-consumption batance and enhancing the yield of metabolites in high-cell density fermentation.

关 键 词：血红蛋白基因 糖多孢红霉菌 CO示差光谱 高密度发酵 

分 类 号：Q753[生物学—分子生物学]