盐酸小檗碱和辛伐他汀对非酒精性脂肪肝大鼠血清脂联素和肝脏脂联素受体表达的影响  

Effects of berberine and simvastatin on serum adiponectin and expression of adiponectin receptor in rats with nonalcoholic fatty liver disease

作　　者：郑贝贝[1] 魏敬[1] 蒋建东[2] 张日东[1] 

机构地区：[1]南京医科大学附属南京第一医院内分泌科,210029 [2]中国医学科学院医药生物技术研究所,北京市100050

出　　处：《实用医学杂志》2010年第15期2701-2704,共4页The Journal of Practical Medicine

基　　金：国家自然科学基金资助项目编号:30271527;江苏省自然科学基金资助项目编号:BK2002142

摘　　要：目的:研究盐酸小檗碱和辛伐他汀对非酒精性脂肪肝大鼠血清脂联素和肝脏脂联素受体表达的影响。方法:32只大鼠随机分为对照组(8只,普通饲料喂养)和模型组(24只,高脂饲料喂养)。高脂饲料喂养建立非酒精性脂肪肝大鼠模型,用盐酸小檗碱和辛伐他汀干预治疗,以辛伐他汀组作阳性对照组,8周后,分别检测大鼠血清肝肾功能、血脂、血清脂联素及大鼠肝脏HE染色和脂联素受体的表达。结果:(1)高脂组血清脂联素水平明显低于对照组(P<0.05);高脂组脂联素受体1(adipoR1)mRNA的表达与正常组比较无明显改变(P>0.05),而脂联素受体2(adipoR2)mRNA的表达与正常组比较显著降低(P<0.01)。(2)盐酸小檗碱和辛伐他汀干预治疗8周后血清脂联素水平较高脂组明显升高(P<0.01),肝脏组织adipoR1-mRNA表达与高脂组相比差异无统计学意义(P>0.05),adipoR2-mRNA表达与高脂组相比差异有统计学意义(P<0.01)。结论:盐酸小檗碱和辛伐他汀都可升高血清脂联素和adipoR2-mRNA的表达,但不影响肝脏adipoR1-mRNA的表达。盐酸小檗碱能有效改善胰岛素抵抗,降低血脂,改善肝功。Objective To investigate the effects of berberine and simvastatin on serum adiponectin and expression of adiponectin receptor in rats with nonalcoholic fatty liver disease （NAFLD）. Methods 32 SD rats were randomized to receive normal diet （control group, n=8） or hlgh-fat diet （NAFLD group, n=24）.The model of NAFLD was established by feeding with high-fat diet. Berberine and simvastatin was used for intervention treatment. On week 8, blood lipids, liver functions were examined, and expression of adiponectin recepter mRNA and protein was detected by reverse quantitative real-time PCR and ELISA, respectively. Results Serum adiponectin level was significantly lower in the NAFLD group than in the control group （P 〈 0.05）. The expression level of adipoR1 mRNA did not differ significantly between the two groups （P 〉 0.05 ） ; but that of adipoR1 mRNA was markedly lower in in the NAFLD group than in the control group （P 〈 0.01 ）. Plasma concentration of adiponectin was elevated after 8- week intervention with berberine and simvastatin （P 〈 0.01）,while there was no significant difference in the expression of adiponectin recepter-1 mRNA （P〉 0.05）.The expression of adiponectin recepter-2 mRNA differed significantly（P 〈 0.01 ）. Conclusions Berberine and simvastatin can increase serum adiponebtin and expression of adiponectin recepter-2 mRNA, but does not affect expression of adiponectin recepter-1 mRNA in the liver.

关 键 词：小檗碱 辛伐他汀 脂联素 受体 脂联素 实时荧光定量PCR 非酒精性脂肪肝 

分 类 号：R285.5[医药卫生—中药学]