宫颈重度鳞状上皮内病变和宫颈癌组织中人乳头瘤病毒感染及基因分型的研究  

Study of Infection of Human Papillomavirus and its Gene Typing in High Grade Squamous Intraepithelial Lesion of Cervix and Cervical Cancer

作　　者：魏颖颖[1] 李劼[1] 王博[1] 闵小佳[1] 曾蓉[1] 

机构地区：[1]湖南师范大学第一附属医院湖南省人民医院

出　　处：《实用妇产科杂志》2010年第7期507-509,共3页Journal of Practical Obstetrics and Gynecology

基　　金：湖南省科技计划资助项目(No.2008SK3042)

摘　　要：目的：探讨人乳头瘤病毒感染及其基因型与宫颈重度鳞状上皮内病变（HSIL）和宫颈癌的关系。方法：采用可检测25种HPV基因型的基因芯片方法分别检测HSIL（23例）和宫颈癌（46例）组织的HPV基因型，计算两组患者宫颈组织中HPV的感染率及各基因型的感染率，比较HPV及其主要基因型与宫颈病变的关系。结果：69例宫颈病变患者中HPV阳性率为98．55％（68／69）；HSIL组单一型别感染率为81．82％，多型别感染率为18．18％，HPV的型别分布以HPVl6、52、58最常见；宫颈癌患者中单一型别感染率为95．45％，多型别感染率为4．55％，HPv的型别分布以HPVl6、58最常见。所检出的HPV型别中以HPVl6的检出率最高。结论：①HPV感染，特别是高危型HPV感染与HSIL和宫颈癌的关系密切；②宫颈病变严重程度可能与HPV基因型多寡无关，而与感染HPV亚型的致病能力密切相关；（9HSIL与宫颈癌感染的HPV基因型呈多样性，且均为高危型感染，其中以HPV16型最常见。Objective：To investigate the relationship of human papilloma virus（HPV） infection and its typ- ing with high-grade squamous intraepithelial lesion（HSIL） and cervical cancer. Methods： HPV gene subtypes were checked in 23 cases of HSIL and 46 cases of cervical cancer by gene chip technique. Infective rate of HPV and its subtypes were calculated. The association of genotype distribution of HPV with different lesions of cervix was explored. Results： HPV infection rate of the 69 samples was 98.55% （68/69） ; the single and multiple subtype gene infection in HSIL were 81.82% and 18. 18%, the most common subtypes were HPV16,52,58; the single and multiple subtype gene infection in cervical cancer were 95.45% and 4.55%, the most common subtypes were HPV16 and HPV58. HPV16 was the most common type in all lesions. Con- clusions：①High risk HPV subtype infection has tight relationship with HSIL and cervical cancer;②The se- verity of cervical lesions may not associate with the quantity of the HPV subtypes, but associate wity the viru- lence of HPV subtypes. ③HPV genotypes are diversed in HSIL and cervical cancer,and all of which are high risk type, among them HPVI6 is the most common one.

关 键 词：人乳头瘤病毒 宫颈重度鳞状上皮内病变 宫颈癌 

分 类 号：R737.33[医药卫生—肿瘤]