Effects of a type-II RNA-binding protein on fatty acid composition in Synechocystis sp. PCC 6803  

作　　者：TANG Qing TAN XiaoMing XU XuDong 

机构地区：[1]State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

出　　处：《Chinese Science Bulletin》2010年第22期2416-2421,共6页

基　　金：supported by the National Natural Science Foundation of China (30825003);the National Basic Research Program of China (2008CB418001);the Knowledge Innovation Project of the Chinese Academy of Sciences (KZCX1-YW-14-1)

摘　　要：In the cyanobacterium Synechocystis 6803, rbp3, a type-II RNA-binding protein gene, is slightly induced by temperature downshift. An rbp3 mutant shows significant reduction in total polyunsaturated fatty acids (PUFA) in membrane lipids. However, the reduction in PUFA has not attained the extent that would significantly affect the growth of the mutant at low temperature. Transcripts of fatty acid desaturase genes desA, desB and desD, and ccr-1, a gene required for growth at 15°C, are significantly reduced in the mutant relative to the wild type, while transcripts of rbp1 (RNA-binding protein 1) and crhR (RNA helicase Light) are not affected. Rbp3 may directly or indirectly affect mRNA levels of certain genes.In the cyanobacterium Synechocystis 6803, rbp3, a type-ⅡRNA-binding protein gene, is slightly induced by temperature downshift. An rbp3 mutant shows significant reduction in total polyunsaturated fatty acids （PUFA） in membrane lipids. However, the reduction in PUFA has not attained the extent that would significantly affect the growth of the mutant at low temperature. Transcripts of fatty acid desaturase genes desA, desB and desD, and ccr-1, a gene required for growth at 15℃, are significantly reduced in the mutant relative to the wild type, while transcripts of rbpl （RNA-binding protein 1） and crhR （RNA helicase Light） are not affected. Rbp3 may directly or indirectly affect mRNA levels of certain genes.

关 键 词：RNA结合蛋白 脂肪酸组成 蓝藻 多不饱和脂肪酸 类型 可持续发展教育 作者 RNA解旋酶 

分 类 号：Q513[生物学—生物化学] TS221[轻工技术与工程—粮食、油脂及植物蛋白工程]