侵染马铃薯的一种短杆状病毒的分离鉴定(英文)  

Identification of a Rod Shaped Virus Infecting Potato

作　　者：杨静华[1] 金萍[1] 马丽杰[1] 庞瑞杰[1] 张鹤龄[1] 

机构地区：[1]内蒙古大学生物学系

出　　处：《内蒙古大学学报（自然科学版）》1999年第2期230-235,共6页Journal of Inner Mongolia University：Natural Science Edition

基　　金：国家自然科学基金

摘　　要：从马铃薯“紫花白”叶片中分离到一种短杆状病毒，病毒粒体直径为１９±１．９ｎｍ，长度在１８０～２２０ｎｍ之间，ＯＤ２６０／２８０比值为１．２３，病毒核酸为ｓｓＲＮＡ，单一组分．用提纯的病毒免疫家兔，制备抗血清，微量沉淀法表明此病毒与ＰＬＲＶ，ＰＶＸ，ＰＶＹ，ＰＶＳ，ＴＭＶ均无血清学关系．病毒分离物可摩擦接种茄科，苋科、藜科植物，并在不同指示植物上引起不同的症状，但只在普通烟上产生系统感染．电镜观察和ＤＡＳ－ＥＬＬＳＡ检测证实此病毒可摩擦回接马铃薯“紫花白”，但不引起明显症状．病毒的稀释限点为１０－４～１０－５，体外存活期为６ｄ，致死温度为８０～８５℃．与目前已报道的侵染马铃薯的各种杆状ＲＮＡ病毒比较分析证明，此病毒是侵染马铃薯的一种新病毒，命名为马铃薯短杆状病毒（Ｐｏｔａｔｏｒｏｄ－ｓｈａｐｅｄＶｉｒｕｓ，ＰＲＶ）．A rod shaped virus was isolated from infected leaves of potato caltivar “Purple flower white” and identified,named Potato rod shaped virus (PRV).The virus particles were (19±1.9) nm in diameter with predominant lengths of 180￣220 nm.The nucleic acid of PRV was isolated and identified as a single stranded RNA which is smaller than TMV RNA.An antiserum against PRV was prepared by immunizing the rabbit.Microprecipitation tests shows that this antiserum did not react with PVX,PVY,PVS,PLRV and TMV.The PRV did not react with antisera against TMV,PVX,PVY,PVS and PLRV.PRV can be transmitted by sap to 10 species of Solanaceae,Amaranthacea and Chenopodiaceae. The infection of potato “Purple flower white” by PRV was confirmed by back inoculation,but infected plants did not show any distinct stmptoms,but the virus particles were found under electron microscope in the sap of infected plant leaves.The experiments on stability of PRV in sap show that DEP 10 -4 ￣10 -5 ,TIP 80￣85 ℃,SIV 6 days at room temperature.This rod shaped virus is different from other reported plant viruses infected potato and similar rod shaped plant viruses.PRV is a new virus infecting potato.

关 键 词：马铃薯 短杆状病毒 分离 鉴定 PRV 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]