HBxAg蛋白反式激活基因5的生物信息学分析及真核表达载体的构建  

Bioinformatics prediction and construction of eukaryotic expression vectors for human gene 5 transactivated by HBxAg

作　　者：蓝贤勇[1] 安纪红 成军[1] 武会娟[1] 张丽娟[1] 陶明亮[1] 袁菊[1] 张黎颖[1] 洪源[1] 毛羽[1] 陈宏[3] 

机构地区：[1]北京地坛医院传染病研究所,北京100011 [2]内蒙古医院感染科 [3]西北农林科技大学动物科技学院

出　　处：《中华实验和临床感染病杂志（电子版）》2007年第4期213-215,共3页Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Edition)

摘　　要：目的对HBxAg蛋白反式激活基因5(XTP5)进行生物信息学分析,并构建其真核表达载体。方法利用生物信息学软件对XTP5基因进行CpG岛、启动子活性、转录终止信号和氨基酸的一、二级结构以及信号肽、跨膜特性和功能基序进行预测。构建XTP5基因的真核表达载体pcDNA3.1-XTP5和pGBKT7-XTP5。结果生物信息学分析发现XTP5基因有200bp左右的CpG岛,有高启动子活性序列、转录终止信号位点,二级结构以Helix为主,存在信号肽的概率为0.0000A,无跨膜区域信号,存在数个功能基序。成功构建了XTP5基因的真核表达载体pcDNA3.1-XTP5和pGBKT7-XTP5。结论为深入研究XTP5结构和功能、进一步探讨乙型肝炎病毒(hepatitis B virus,HBV)发病机制创造了条件。Objective To analyze XTP5 gene（minor histocompatibility antigen HA-8） by bioinformatics and to construct eukaryotic expression vectors.Methods Bioinformatics software was applied to analyze XTP5 gene as followings：CpG island,promoter activity,transcription terminat signal,the first and second structure of amino acids,signal peptide,prediction for characteristics of transmembrane and functional motif.Moreover,eukaryotic expression vectors of XTP5 gene were contructed.Results CpG island was found with a length of 200 bp.High promoter activity and transcription terminat signal site was also found.The main second structure of amino acids is Helix.The probability of having signal peptide is 0.0000A.Several functional motifs were found but no transmembrane signal was found.The eukaryotic expression recombinants（pcDNA3.1-XTP5 and pGBKT7-XTP5） were constructed successfully.Conclusions This study create conditions for deeply studying the pathogenesis of HBV.

关 键 词：乙型肝炎病毒X蛋白 XTP5基因 次要组织相容性抗原基因 生物信息学 表达载体 

分 类 号：R346[医药卫生—基础医学]