胆碱受体拮抗剂在小鼠骨髓细胞中的C──有丝分裂效应研究  

C-Mitosis Induction by Antagonists to Cholinergic Receptorin Mouse Bone Marrow Cells

作　　者：和智君[1] 梁子卿[1] 李晓琼[1] 汪旭[1] 

机构地区：[1]云南师范大学生命科学系,昆明650092

出　　处：《云南师范大学学报（自然科学版）》1999年第1期1-3,共3页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金;云南省国际合作基金

摘　　要：本文分别对毒蕈碱型、烟碱型乙酰胆碱受体拮抗剂阿托品和筒箭毒碱在小鼠骨髓细胞中的Ｃ──有丝分裂效应做了初步探讨，从而为该类化合物诱发非整倍体的可能性和途径机制研究提供初步实验资料。实验用阿托品和筒箭毒碱最高剂量为７０％ＬＤ５０。结果发现，阿托品在最高剂量组（５．２ｍｇ／ｋｇ）分别导致小鼠骨髓细胞有过分裂指数和Ｃ──有丝分裂细胞频率显著升高（Ｐ＜０．００１，Ｐ＜０．０５＝、有丝分裂后期细胞频率显著下降（Ｐ＜０．００１＝。在受试剂量范围内，未发现筒箭毒碱具有类似效应（０．０６３－０．１２５ｍｇ／ｋｇ）。毒蕈碱型乙酰胆碱受体拮抗剂阿托品在小鼠骨髓细胞中的Ｃ──有丝分裂效应提示乙酰胆碱受体的正常功能与细胞有丝分裂的真实、准确性有一定关系。The study investigated the C-mitotic effects in mouse bone marrow cells induced by atropineand d-tubocuraine, which are antagonists to muscarinic and nicotinic receptors, respectively. The dosesused in the experiments were 70% LD50. The results showed that the atropine(highest dose , 5. 2mg/kg)significantly induced increases of mitotic index (MI) and colchicine-mitotic frequency (P<0. 001 , P<0.05) , the frequency of anaphase was decreased (P<0. 001 ). It is reasonable to hypothesize that acetyl-choline and its receptor take part in the regulation of mitosis in mouse bone marrow cells. As C-mitotic ef-fects is a kind of sign for aneuploidy induction.

关 键 词：胆碱受体拮抗剂 有丝分裂 骨髓细胞 C-有丝分裂 

分 类 号：R329.25[医药卫生—人体解剖和组织胚胎学] Q343.244[医药卫生—基础医学]