调控食管癌EC9706细胞DNA聚合酶β表达的初步研究  

Regulation of DNA polymerase β expression in esophageal carcinoma cell line EC9706

作　　者：王涛[1] 董子明[2] 赵国强[2] 

机构地区：[1]河南省人民医院医学遗传研究所,河南郑州450003 [2]郑州大学基础医学院,河南郑州450052

出　　处：《中国病理生理杂志》2010年第8期1464-1467,共4页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30471952)

摘　　要：目的:观察DNA聚合酶β(DNA polβ)对食管癌EC9706细胞生物学特性的影响。方法:以pSINsi-hU6为载体构建DNA polβ靶向的siRNA表达质粒,转染EC9706细胞,G418筛选。流式细胞术和裸鼠体内成瘤实验检测细胞增殖情况;实时荧光定量PCR检测细胞DNA polβmRNA的表达。结果:与空载体组和对照组相比,实验组1和实验组2(转染siRNA重组表达质粒)DNA polβmRNA的表达显著下降(P<0.05),DNA polβ高表达组中mRNA的表达显著升高(P<0.05);实验组1、实验组2和DNA polβ高表达组细胞周期S期比例显著增加(P<0.05),肿瘤重量亦显著增加(P<0.05)。结论:DNA polβ的高表达和过低表达,都会增加细胞的遗传不稳定性,可能在食管癌的发生中起一定作用。AIM： To investigate the influence of DNA polymerase β（DNA polβ） on the biological characteristics of esophageal carcinoma cells.METHODS： siRNA expression plasmid targeting DNA polβ was constructed and transfected into EC9706 cells.The positive colonies were screened by G418 selection.The proliferation of EC9706 cells was detected by flow cytometry and nude mice xenograft model.The expression of DNA polβ was detected by real-time PCR.RESULTS： Compared to empty vector group and control group,the expression of DNA polβ was significantly reduced in the cells transfected with siRNA expression plasmid pSINsi-hU6-siRNA polβ288（experimental group 1） and the cells transfected with siRNA expression plasmid pSINsi-hU6-siRNA polβ814（experimental group 2）.The expression of DNA polβ was significantly increased in DNA polβ over-expression group（P0.05）.The proportion of the cells in S phase increased significantly in experimental group 1,experimental group 2 and DNA polβ over-expression group（P0.05）.The weight of tumors increased significantly（P0.05）.CONCLUSION： The over-expression and extremely low expression of DNA polβ may increase genetic instability of the cells and play a role in the occurrence of esophageal carcinoma.

关 键 词：食管肿瘤 DNA聚合酶Β RNA干扰 SIRNA表达载体 

分 类 号：R735.1[医药卫生—肿瘤]