NGF对乳腺癌细胞系MDA-MB-231和MCF-7增殖和存活的影响  

Effect of nerve growth factor on proliferation and survival of breast cancer cell line MDA-MB-231 and MCF-7

作　　者：胡梦娜[1] 邹丽娟[1] 袁崇刚[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062

出　　处：《中国病理生理杂志》2010年第8期1483-1488,共6页Chinese Journal of Pathophysiology

基　　金：上海市国际科技合作基金资助项目(No.075407059)

摘　　要：目的:研究神经生长因子(NGF)对乳腺癌细胞系MDA-MB-231和MCF-7细胞增殖与存活的影响。方法:运用免疫荧光方法检测NGF及其受体酪氨酸蛋白激酶A(TrkA)的表达;运用酶联免疫吸附测定(ELISA)方法检测细胞的NGF自分泌情况;运用蛋白质印迹(Western blotting)方法检测TrkA蛋白的表达情况;运用NGF阻断剂Ro 08-2750对细胞进行NGF剥夺,通过单核细胞直接细胞毒性测定法(MTT)检测细胞增殖的情况;运用流式细胞仪检测细胞凋亡情况以及细胞周期分布的变化。结果:2株乳腺癌细胞系均表达NGF及其受体TrkA,NGF阻断剂Ro 08-2750能够明显抑制2种细胞的增殖,并具有剂量依赖性;流式细胞仪显示Ro 08-2750处理的MDA-MB-231细胞和MCF-7细胞的S期细胞比例明显增加,G2/M期细胞比例明显降低,MDA-MB-231细胞出现凋亡峰。结论:NGF剥夺明显抑制乳腺癌细胞系MDA-MB-231和MCF-7的增殖,并引起MDA-MB-231细胞凋亡。AIM： To study the effect of nerve growth factor（NGF） on the proliferation and survival of breast cancer cell lines MDA-MB-231 and MCF-7.METHODS： Two breast cancer cell lines MDA-MB-231 and MCF-7 were used in the experiment.The expression of NGF and its receptor tyrosine kinase A（TrkA） was determined by the method of immunofluorescence.The NGF autocrine was detected by the method of enzyme linked immunosorbent assay（ELISA）.The expression of TrkA was measured by Western blotting.The effect of NGF blocking agent Ro 08-2750 on the proliferation of the cells was evaluated by MTT assay（mono-nuclear cell direct cytotoxicity assay）.The cell apoptosis and the change of the cell cycle after exposed to Ro 08-2750 were observed by flow cytometry.RESULTS： Both cell lines expressed NGF and TrkA.Ro 08-2750 inhibited the proliferation of both cell lines in a dose-dependent manner.According to the results of flow cytometry,the S-phase cells in both cell lines increased but the G2/M-phase cells decreased after treated with Ro 08-2750.An apoptotic peak occurred in MDA-MB-231 cells.CONCLUSION： The results suggest that proliferation of MDA-MB-231 and MCF-7 cell lines is dependent on NGF.NGF promotes the survival of MDA-MB-231 cells.

关 键 词：神经生长因子 酪氨酸蛋白激酶A MDA-MB-231细胞 MCF-7细胞 

分 类 号：R363[医药卫生—病理学]