阿司匹林增强TRAIL诱导的HepG-2细胞凋亡  

Aspirin enhances TRAIL-induced apoptosis in HepG-2 cells

作　　者：戚之琳[1] 李璐[1] 汪茗[1] 毕富勇[1] 

机构地区：[1]皖南医学院生物化学教研室,安徽芜湖241001

出　　处：《中国病理生理杂志》2010年第8期1555-1558,共4页Chinese Journal of Pathophysiology

基　　金：安徽省高校青年教师资助计划资助项目(No.2008jq1152)

摘　　要：目的:研究阿司匹林能否增强肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导的HepG-2细胞凋亡,并初步探讨其作用机制。方法:HepG-2细胞放入含15%胎牛血清的DMEM培养液中培养,改良苯酚-品红染色观察细胞形态变化,MTT法检测细胞增殖程度,TUNEL法检测细胞凋亡指数,RT-PCR检测细胞中survivinmRNA的表达水平,FCM检测细胞凋亡率和细胞周期。结果:不同浓度的阿司匹林联合TRAIL实验组细胞增殖抑制率明显高于空白对照组及TRAIL和阿司匹林单独用药组,且细胞凋亡指数也明显提高,凋亡率与阿司匹林的浓度呈现剂量依赖关系,联合用药组凋亡相关基因survivin的表达与空白对照组和TRAIL和阿司匹林单用组相比明显下调。结论:阿司匹林能够增强TRAIL诱导的HepG-2细胞凋亡,其作用机制可能与survivin基因的表达下调有关。AIM： To investigate the enhancing effect of aspirin on the HepG-2 cell apoptosis induced by tumor necrosis factor-related apoptosis-inducing ligand（TRAIL） and its underlying mechanism.METHODS： HepG-2 cells were cultured in DMEM medium with 15% fatal bovine serum.Modified carbol fuchsin staining was used to observe the cellular morphology.Cell proliferation was measured by MTT.The method of terminal deoxynucleotide transferase-mediated dUTP nick end labeling（TUNEL） was used to examine the apoptotic index of the cells.RT-PCR was applied to detect survivin gene expression.The apoptotic rate and cell cycle were determined by flow cytometry.RESULTS： Aspirin at different concentrations and TRAIL inhibited the proliferation of HepG-2 cells and the cell apoptotic index was obviously higher than that in control group.Aspirin increased the apoptotic rate in a dose-dependent manner.Aspirin at different concentrations and TRAIL significantly down-regulated the mRNA level of survivin.CONCLUSION： Aspirin enhances the apoptosis of HepG-2 cells induced by TRAIL and the mechanism may be related to down-regulating the mRNA expression of survivin.

关 键 词：肿瘤坏死因子相关凋亡诱导配体 阿司匹林 HEPG-2细胞 细胞凋亡 基因SURVIVIN 

分 类 号：R363[医药卫生—病理学]