CML病人外周血T细胞CD3基因表达模式变化  

Change of Expression Pattern of CD3 Genes in Peripheral Blood T-Cells from CML Patients

作　　者：杨力建[1] 陈少华[1] 王亮[2] 陈思[1] 郁志[2] 卢育洪[2] 李扬秋[1,3] 

机构地区：[1]暨南大学医学院血液病研究所,广东广州510632 [2]暨南大学附属第一医院血液科,广东广州510632 [3]暨南大学再生医学教育部重点实验室,广东广州510632

出　　处：《中国实验血液学杂志》2010年第4期937-941,共5页Journal of Experimental Hematology

基　　金：广东省自然科学基金重点项目(编号9251063201000001);广东省医学科研基金(编号A2008341)

摘　　要：在我们前期研究发现慢性髓系白血病(CML)病人中T细胞受体信号传导分子CD3ζ基因表达下调的基础上,本研究进一步分析病人外周血中全部4种CD3γδεζ基因的表达模式特点,以深入了解病人的T细胞免疫中信号转导的变化。利用SYBR GreenⅠ实时荧光定量RT-PCR检测17例初诊慢性期CML病人外周血单个核细胞中CD3γ、δ、ε和ζ链的表达水平,以β2微球蛋白基因(β2MG)作为内参照,采用相对定量公式:2-△Ct×100%,计算CD3分子各亚单位的相对mRNA表达量,以17例健康成人外周血作为对照。结果表明:CML中CD3γ、δ、ε基因表达水平分别为2.344%,0.515%和3.516%(中位数),与健康对照组比较(3.093%,0.853%和2.302%)并没有显著性差异(p=0.072,p=0.190,p=0.615),而CML中CD3ζ链基因的表达水平(0.395%)则明显低于健康对照组(1.538%)(p<0.001)。在CML中4种CD3分子表达水平模式依次为CD3ε>CD3γ>CD3δ>CD3ζ,而对照组中则为CD3γ>CD3ε>CD3ζ>CD3δ。结论:本研究首先提供了CML病人中CD3各基因表达模式情况,显示以CD3ζ为主的TCR信号分子的表达缺陷,4种CD3链分子的表达水平模式也发生变化。Our previous finding showed that down-regulation of CD3ζ gene was detected in patients with chronic my eloid leukemia（CML）. In order to further elucidate the feature of T cell immune status in the signal transduction in CML patients, the expression patterns of all 4 CD3 genes were characterized in peripheral blood of patients, the expression levels of CD3γ, δ, ε and ξ chain genes were detected by real time qPCR with SYBR Green I staining in peripheral blood mononuclear cells （PBMNCs） from 17 cases of de novo CML patients in chronic phase and 17 cases of healthy individuals, the β2-microglobulin gene was used as an internal reference, and the mRNA expression level of each CD3 gene was evaluated by the 2 - △Cr × 100% method. The results showed that the median expression levels of CD3γ, δ and ε genes （2. 344% ,0. 515% and 3. 516% ） in CML patients were not significantly different from healthy individuals （p =0. 072, p =0. 190,p = 0. 615, respectively）, while the expression level of CD3ζ gene in PBMNCs from CML patients （0. 395% ） was lower than that from healthy individuals （ 1. 538% ） （p 〈 0. 001 ）. The expression patterns of 4 CD3 genes in proper order were CD3ε 〉 CD3γ 〉 CD3δ 〉 CD3ζ in CML group, in contrast, the expression patterns were pres- ented as CD3γ〉 CD3ε 〉 CD3δ 〉 CD3ζ in healthy group. It is concluded that the present study characterized the expression pattern of CD3γ, δ, ε and ζchain genes in CML patients, lower expression of CD3ζ is the feature of TCR signal transduction immunodeficiency and the expression patterns of 4 CD3 genes are changed in CML patients.

关 键 词：慢性髓系白血病 CD3链基因 基因表达 免疫缺陷 

分 类 号：R733.72[医药卫生—肿瘤]