胞壁酰二肽对儿童急性白血病骨髓树突状细胞体外扩增的影响  

Effect of Muramyl Dipeptide on Proliferation of Dendritic Cells Derived from Children Acute Leukemia Bone Marrow In Vitro

作　　者：李晓玲[1] 孙立荣[1] 

机构地区：[1]青岛大学医学院附属医院小儿血液科

出　　处：《中国实验血液学杂志》2010年第4期963-966,共4页Journal of Experimental Hematology

摘　　要：本研究旨在探讨胞壁酰二肽(muramyl dipeptide,MDP)对急性白血病儿童骨髓树突状细胞(DC)体外扩增及成熟的影响。采用Ficoll-Hypaque法分离急性白血病患儿骨髓单个核细胞。实验分为4组:对照组DC以含10%FCS的RPMI1640培养液培养;实验1组:DC单独应用胞壁酰二肽;培养实验2组:DC用rhGM-CSF+rhIL-4+rhTNF-α培养;实验3组:DC用rhGM-CSF+rhIL-4+rhTNF-α+MDP培养,分别诱导培养DC。每日光学显微镜下观察细胞生长情况及细胞形态,培养8天后,计数各组细胞数量,并应用流式细胞术检测各组细胞免疫表型。结果表明:①实验各组均得到一定数量典型的DC,对照组DC数(0.85±0.23)×105/L,而实验1组、实验2组、实验3组DC数分别为(2.31±0.24)、(3.26±0.37)及(4.16±0.34)×105/L,均明显高于对照组(p均<0.01);实验1组DC数低于2组及3组(p均<0.01);实验3组DC数明显高于2组(p<0.01)。②对照组、实验1组、实验2组及实验3组的HLA-DR细胞比例分别为19.98±3.74、37.24±4.32、58.81±2.08、77.48±5.57,实验1组明显高于对照组(p<0.01),而低于实验2组、实验3组(p均<0.01);实验3组明显高于2组(p<0.01)。对照组、实验1组、实验2组及实验3组的CD1a细胞比例分别为11.46±2.43、28.71±6.64、46.92±4.78、57.03±3.07,实验1组明显高于对照组(p<0.01),而低于实验2组、实验3组(p均<0.01);实验3组明显高于2组(t=3.98,p<0.01)。对照组、实验1组、实验2组及实验3组的CD83细胞比例分别为(13.05±5.70)%、(36.32±5.61)%、(54.95±7.83)%、(75.70±6.67)%,实验1组明显高于对照组(p<0.01),而低于实验2组、实验3组(p均<0.01);实验3组明显高于2组(p<0.01)。结论:MDP不仅对急性白血病患儿骨髓DC有扩增作用,而且能促进其成熟,并能协同细胞因子促进DC增殖和成熟,但MDP促进DC增殖的能力弱于其与细胞因子的联合作用。The aim of this study was to explore the effect of muramyl dipeptide （MDP） on proliferation of dentritic ceils （DCs） from bone marrow of children with acute leukemia in vitro. The mononuclear cells were isolated from bone marrow of children with acute leukemia to induce dendritic cells. The experiment was divided into 4 groups. The control group ： MNC ＋ RPMI 1640 medium; test group 1 ： MNC ＋ MDP; test group 2 ： MNC ＋ rhGM-CSF ＋ IL-4 ＋ rhTNFot; test group 3 ：MNC ＋ rhGM-CSF ＋ IL-4 ＋ rhTNFα ＋ MDP. The growth of DCs was observed by inverted microscope every day; the number of DCs in different groups were counted, the immumophenotypes of DCs were detected by flow cytometry on day 8 of culture. The results indicated that a certain number of typical DCs could be detected in all experimental groups. The DC number in control and 3 test groups were （0.85 ±0.23） × 10^5/L, （2.31±0.24） × 10^5/ L, （3.26 ± 0.37 ）× 10^5/L and （4.16 ± 0.34 ） x 10^5/L, respectively, among which DC number is in all 3 test groups were higher than that in control group （p 〈0.01 ）, the DC number in test group 1 was lower than that in test groups 2 and 3 （p 〈 0.01 ）, while it in test group 3 was higher than that in test group 2 （p 〈 0.01 ）. The percentages of HLA-DR in control, test group 1,2 and 3 were 19.98 ± 3.74, 37.24 ± 4.32, 58.81 ± 2.08 and 77.48 ±5.57 respectively; the percentages of CD1 a and CD83 in control, test group 1, 2 and 3 were 11.46 ± 2.43, 28.71± 6.64, 46.92±4.78 and 57.03 ± 3.07, as well as 13.05±5.70, 36.32 ± 5.61,54.95 ± 7.83 and 75.70± 6.67 respectively. The comparison of HLA-DR,CDla and CD83 levels in control and test group 1, 2 showed that their results were consistent with results of DC numbers. It is concluded that MDP not only promotes the proliferation of DCs derived from bone marrow of children with acute leukemia in vitro, cooperates with rhGM-CSF, rhlL-4 and rhTN-CSF in promoting of the proliferation and maturation of DCs, while the p

关 键 词：胞壁酰二肽 骨髓树突状细胞 急性白血病 细胞扩增 

分 类 号：R733.71[医药卫生—肿瘤] R979.1[医药卫生—临床医学]